UM
Affiliated with RCfalse
Status已發表Published
The metabolic footprint during adipocyte commitment highlights ceramide modulation as an adequate approach for obesity treatment
Hou,Weilong1; Chen,Qiang1; Wang,Haitao1,2,3,4; Qiu,Pengxiang1; Lyu,Xueying1; Chen,Weiping5; Chua,Melvin L.K.2,3,4; Chinn,Y. Eugene6; Deng,Chu Xia1; Wang,Ruihong1,7
2020
Source PublicationEBioMedicine
Volume51
Abstract

Background: Metabolic modulation is capable of maintaining cell potency, regulating niche homeostasis, or determining cell fate. However, little is known regarding the metabolic landscape during early adipogenesis or whether metabolic modulation could be a potential approach for obesity treatment.

Methods: The metabolic footprint during adipocyte commitment was evaluated by metabolomics analysis in mouse embryonic fibroblasts (MEFs). The role of apoptosis induced by ceramide and how ceramide is regulated were evaluated by omics analysis in vitro, human database and the adipocyte-specific Sirt1 knockout mouse.

Findings: The metabolic footprint showed that a complicated diversity of metabolism was enriched as early as 3 h and tended to fluctuate throughout differentiation. Subsequently, the scale of these perturbed metabolic patterns was reduced to reach a balanced state. Of high relevance is the presence of apoptosis induced by ceramide accumulation, which is associated with metabolic dynamics. Interestingly, apoptotic cells were not merely a byproduct of adipogenesis but rather promoted the release of lipid components to facilitate adipogenesis. Mechanistically, ceramide accumulation stemming from hydrolysis and the de novo pathway during early adipogenesis is regulated by Sirt1 upon epigenetic alterations of constitutive Histone H3K4 methylation and H3K9 acetylation.

Interpretation: The metabolic footprint during adipocyte commitment highlights that apoptosis induced by ceramide is essential for adipogenesis, which is reversed by suppression of Sirt1. Therefore, Sirt1 may constitute a target to treat obesity or other ceramide-associated metabolic syndromes. Published by Elsevier B.V.

KeywordAdipogenesis Apoptosis Ceramide Epigenetic Modification Metabolic Landscape Metabolomics Obesity
DOI10.1016/j.ebiom.2019.102605
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaGeneral & Internal Medicine ; Research & Experimental Medicine
WOS SubjectMedicine, General & Internal ; Medicine, Research & Experimental
WOS IDWOS:000512904900041
Fulltext Access
Citation statistics
Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorDeng,Chu Xia; Wang,Ruihong
Affiliation1.Faculty of Health Sciences,University of Macau,Macau SAR,China
2.Division of Radiation Oncology,National Cancer Centre Singapore,Singapore
3.Division of Medical Sciences,National Cancer Centre Singapore,Singapore
4.Oncology Academic Programme,Duke-NUS Medical School,Singapore
5.National Institute of Diabetes and Digestive and Kidney Diseases,Bethesda,20892,United States
6.Institute of Biology and Medical Sciences,Soochow University School of Medicine,Suzhou,199# Ren'ai Road,215123,China
7.Center for Cancer Research,Nation Cancer Institute,National Institutes of Health,Bethesda,20892,United States
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Hou,Weilong,Chen,Qiang,Wang,Haitao,et al. The metabolic footprint during adipocyte commitment highlights ceramide modulation as an adequate approach for obesity treatment[J]. EBioMedicine,2020,51.
APA Hou,Weilong,Chen,Qiang,Wang,Haitao,Qiu,Pengxiang,Lyu,Xueying,Chen,Weiping,Chua,Melvin L.K.,Chinn,Y. Eugene,Deng,Chu Xia,&Wang,Ruihong.(2020).The metabolic footprint during adipocyte commitment highlights ceramide modulation as an adequate approach for obesity treatment.EBioMedicine,51.
MLA Hou,Weilong,et al."The metabolic footprint during adipocyte commitment highlights ceramide modulation as an adequate approach for obesity treatment".EBioMedicine 51(2020).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Hou,Weilong]'s Articles
[Chen,Qiang]'s Articles
[Wang,Haitao]'s Articles
Baidu academic
Similar articles in Baidu academic
[Hou,Weilong]'s Articles
[Chen,Qiang]'s Articles
[Wang,Haitao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Hou,Weilong]'s Articles
[Chen,Qiang]'s Articles
[Wang,Haitao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.