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Exploring the Potential of Antibiotic Production From Rare Actinobacteria by Whole-Genome Sequencing and Guided MS/MS Analysis
Hu,Dini1,2; Sun,Chenghang3; Jin,Tao4; Fan,Guangyi4; Mok,Kai Meng2; Li,Kai1; Lee,Simon Ming Yuen5
2020-07-15
Source PublicationFrontiers in Microbiology
ISSN1664-302X
Volume11Pages:1540
Abstract

Actinobacteria are well recognized for their production of structurally diverse bioactive secondary metabolites, but the rare actinobacterial genera have been underexploited for such potential. To search for new sources of active compounds, an experiment combining genomic analysis and tandem mass spectrometry (MS/MS) screening was designed to isolate and characterize actinobacterial strains from a mangrove environment in Macau. Fourteen actinobacterial strains were isolated from the collected samples. Partial 16S sequences indicated that they were from six genera, including Brevibacterium, Curtobacterium, Kineococcus, Micromonospora, Mycobacterium, and Streptomyces. The isolate sp.01 showing 99.28% sequence similarity with a reference rare actinobacterial species Micromonospora aurantiaca ATCC 27029 was selected for whole genome sequencing. Organization of its gene clusters for secondary metabolite biosynthesis revealed 21 clusters encoded to antibiotic production, which is higher than other Micromonospora species. Of the genome-predicted antibiotics, kanamycin was found through guided MS/MS analysis producible by the M. aurantiaca strain for the first time. The present study highlighted that genomic analysis combined with MS/MS screening is a promising method to discover potential of antibiotic production from rare actinobacteria.

KeywordAntibiotics Mangroves Mass Spectrometry Rare Actinobacteria Whole-genome Sequencing
DOI10.3389/fmicb.2020.01540
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000561774800001
PublisherFRONTIERS MEDIA SA, AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE CH-1015, SWITZERLAND
Scopus ID2-s2.0-85089351786
Fulltext Access
FWCI0.7343933
Citation statistics
Document TypeJournal article
CollectionDEPARTMENT OF PHARMACEUTICAL SCIENCES
Faculty of Science and Technology
Institute of Chinese Medical Sciences
DEPARTMENT OF CIVIL AND ENVIRONMENTAL ENGINEERING
THE STATE KEY LABORATORY OF QUALITY RESEARCH IN CHINESE MEDICINE (UNIVERSITY OF MACAU)
Corresponding AuthorLi,Kai; Lee,Simon Ming Yuen
Affiliation1.School of Ecology and Nature Conservation,Beijing Forestry University,Beijing,China
2.Department of Civil and Environmental Engineering,Faculty of Science and Technology,University of Macau,Macao
3.Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China
4.Beijing Genomics Institute,Shenzhen,China
5.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macao
First Author AffilicationFaculty of Science and Technology
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Hu,Dini,Sun,Chenghang,Jin,Tao,et al. Exploring the Potential of Antibiotic Production From Rare Actinobacteria by Whole-Genome Sequencing and Guided MS/MS Analysis[J]. Frontiers in Microbiology,2020,11:1540.
APA Hu,Dini,Sun,Chenghang,Jin,Tao,Fan,Guangyi,Mok,Kai Meng,Li,Kai,&Lee,Simon Ming Yuen.(2020).Exploring the Potential of Antibiotic Production From Rare Actinobacteria by Whole-Genome Sequencing and Guided MS/MS Analysis.Frontiers in Microbiology,11,1540.
MLA Hu,Dini,et al."Exploring the Potential of Antibiotic Production From Rare Actinobacteria by Whole-Genome Sequencing and Guided MS/MS Analysis".Frontiers in Microbiology 11(2020):1540.
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