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13-Methylberberine improves endothelial dysfunction by inhibiting NLRP3 inflammasome activation via autophagy induction in human umbilical vein endothelial cells
Peng,Zhihua1,2,3; Zhan,Hong1; Shao,Yijia2; Xiong,Yan1; Zeng,Lijin1,2,3; Zhang,Cong1,2,3; Liu,Zhihao1; Huang,Zhenhua1; Su,Huanxing4; Yang,Zhen1,2,3
2020-01-22
Source PublicationChinese Medicine
ISSN1749-8546
Volume15Issue:1
Abstract

Background: Atherosclerosis, the underlying cause of the majority of cardiovascular diseases, is a lipid-driven, inflammatory disease of the large arteries. Atherosclerotic cardiovascular disease (ASCVD) threatens human lives due to high morbidity and mortality. Many studies have demonstrated that atherosclerosis is accelerated via activation of the NLRP3 inflammasome. The NLRP3 inflammasome plays a critical role in the development of vascular inflammation and atherosclerosis. In atherosclerotic plaques, excessive generation of reactive oxygen species (ROS) activates the NLRP3 inflammasome. 13-Methylberberine (13-MB) is a newly synthesized compound used in traditional Chinese medicine that has outstanding antibacterial, antitumor, and antiobesity activities, especially anti-inflammatory activity. However, the role of 13-MB in atherosclerosis needs to be explored. Methods: CCK-8 assays and flow cytometry were conducted to determine the cell viability and apoptotic profiles of human umbilical vein endothelial cells (HUVECs) treated with 13-MB. Carboxy-DCFH-DA and JC-10 assays were used to measure ROS and determine mitochondrial membrane potential. Western blot analysis was performed to investigate proteins that are associated with the NLRP3 inflammasome and autophagy. ELISA was used to detect and quantify inflammatory cytokines related to the NLRP3 inflammasome. Transfection and confocal microscopy were conducted to observe autophagy. Results: Pretreatment with 13-MB markedly reduced cytotoxicity and apoptosis, as well as intracellular ROS production, in HO-induced HUVECs. Moreover, 13-MB showed a protective effect in maintaining mitochondrial membrane potential. 13-MB also suppressed NLRP3 inflammasome activation and promoted autophagy induction in HUVECs. Conclusion: 13-MB exerts cytoprotective effects in an HO-induced cell injury model by inhibiting NLRP3 inflammasome activation via autophagy induction in HUVECs. These anti-inflammatory and autophagy induction activities may provide valuable evidence for further investigating the potential role of 13-MB in atherosclerosis.

Keyword13-methylberberine Anti-inflammatory Atherosclerosis Autophagy Inducer Nlrp3 Inflammasome
DOI10.1186/s13020-020-0286-1
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS SubjectIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000521211700001
Scopus ID2-s2.0-85078782703
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorSu,Huanxing; Yang,Zhen
Affiliation1.Division of Emergency Medicine,Department of Emergency Intensive Care Unit,First Affiliated Hospital,Sun Yat-Sen University,Guangzhou,510080,China
2.Department of Cardiology,First Affiliated Hospital,Sun Yat-Sen University,Guangzhou, Guangdong,510080,China
3.Key Laboratory on Assisted Circulation,Ministry of Health,First Affiliated Hospital,Sun Yat-Sen University,Guangzhou,510080,China
4.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macao
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Peng,Zhihua,Zhan,Hong,Shao,Yijia,et al. 13-Methylberberine improves endothelial dysfunction by inhibiting NLRP3 inflammasome activation via autophagy induction in human umbilical vein endothelial cells[J]. Chinese Medicine,2020,15(1).
APA Peng,Zhihua,Zhan,Hong,Shao,Yijia,Xiong,Yan,Zeng,Lijin,Zhang,Cong,Liu,Zhihao,Huang,Zhenhua,Su,Huanxing,&Yang,Zhen.(2020).13-Methylberberine improves endothelial dysfunction by inhibiting NLRP3 inflammasome activation via autophagy induction in human umbilical vein endothelial cells.Chinese Medicine,15(1).
MLA Peng,Zhihua,et al."13-Methylberberine improves endothelial dysfunction by inhibiting NLRP3 inflammasome activation via autophagy induction in human umbilical vein endothelial cells".Chinese Medicine 15.1(2020).
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