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Aqueous Extract of Salvia miltiorrhiza Bunge-Radix Puerariae Herb Pair Attenuates Osteoporosis in Ovariectomized Rats Through Suppressing Osteoclast Differentiation
Qin,Huan1; Zhao,Wenwen1,2; Jiao,Yang3; Zheng,Haoyi1; Zhang,Hao1; Jin,Jingyu4; Li,Qiu5; Chen,Xiuping1,2; Gao,Xia6; Han,Yantao1
2021-01-21
Source PublicationFrontiers in Pharmacology
ISSN1663-9812
Volume11
Abstract

Traditional herb pair Salvia miltiorrhiza Bunge-Radix Puerariae (DG) owns various biological activities including anti-inflammatory and anti-oxidative stress. Oxidative stress is one high-risk factor for osteoporosis, then effect of DG on osteoporosis and underlying mechanisms was explored both in vivo and in vitro. Firstly, the predication from network pharmacology hinted that DG has the potential for ameliorating osteoporosis. Consistent with predication, DG significantly restored bone loss and deficiency of type II collagen, decreased TRAP and Cathepsin K positive areas in femur. Meanwhile it improved important characteristics of microarchitectural deterioration of tissue, reduced the numbers of NFATc1-positive osteoclast in the vertebra as well as decreased the serum osteoclast-specific cytokine RANKL and OPG release in OVX rats exhibiting its protective effect against osteoporosis. In vitro, DG noticeably decreased osteoclastic-special marker protein expressions of RANK, c-Fos and NFATc1. Furthermore, autophagy pathway p62/LC3B, ROS production and NF-κB were all activated by RANKL stimulation and blocked by DG pretreatment. Moreover, autophagy inhibitors, ROS scavenger, Ca chelator and NF-κB inhibitor remarkably suppressed c-Fos and NFATc1 expressions. Taken together, DG may ameliorate osteoporosis by regulating osteoclast differentiation mediated by autophagy and oxidative stress. This study provided a mechanistic basis for DG treating osteoporosis and offered a safe dose for DG in preventing and improving bone diseases.

KeywordAutophagy Osteoclast Differentiation Osteoporosis Oxidative Stress Salvia Miltiorrhiza Bunge-radix Puerariae
DOI10.3389/fphar.2020.581049
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000614862200001
Scopus ID2-s2.0-85100681507
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorHan,Yantao
Affiliation1.School of Basic Medical Sciences,Qingdao University,Qingdao,China
2.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macao,China
3.Department of Biomedical Engineering City,University of Hong Kong,Hong Kong SAR,China
4.Department of Pharmacology,School of Pharmacy,Qingdao University,Qingdao,China
5.College of Chemistry and Pharmaceutical Sciences,Qingdao Agricultural University,Qingdao,China
6.Qingdao Central Hospital,The Second Affiliated Hospital of Qingdao University,Qingdao,China
Recommended Citation
GB/T 7714
Qin,Huan,Zhao,Wenwen,Jiao,Yang,et al. Aqueous Extract of Salvia miltiorrhiza Bunge-Radix Puerariae Herb Pair Attenuates Osteoporosis in Ovariectomized Rats Through Suppressing Osteoclast Differentiation[J]. Frontiers in Pharmacology,2021,11.
APA Qin,Huan,Zhao,Wenwen,Jiao,Yang,Zheng,Haoyi,Zhang,Hao,Jin,Jingyu,Li,Qiu,Chen,Xiuping,Gao,Xia,&Han,Yantao.(2021).Aqueous Extract of Salvia miltiorrhiza Bunge-Radix Puerariae Herb Pair Attenuates Osteoporosis in Ovariectomized Rats Through Suppressing Osteoclast Differentiation.Frontiers in Pharmacology,11.
MLA Qin,Huan,et al."Aqueous Extract of Salvia miltiorrhiza Bunge-Radix Puerariae Herb Pair Attenuates Osteoporosis in Ovariectomized Rats Through Suppressing Osteoclast Differentiation".Frontiers in Pharmacology 11(2021).
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