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Autophagy modulator scoring system: a user-friendly tool for quantitative analysis of methodological integrity of chemical autophagy modulator studies
Dong,Yu1; Hu,Yuanjia1; Sarkar,Sovan2; Zong,Wei Xing3; Li,Min4; Feng,Du5; Song,Ju Xian6,7; Li,Min7; Medina,Diego L.8,9; Tan,Jieqiong10; Zhang,Zhuohua11; Yue,Zhenyu12; Lu,Jia Hong1
Source PublicationAutophagy

Over the past 20 years (1999–2019), we have witnessed a rapid increase in publications involving chemical macroautophagy/autophagy modulators. However, an overview of the methodologies used in these studies is still lacking, and methodology flaws are frequently observed in some reports. To provide an objective and quantitative analysis of studies involving autophagy modulators, we present an Autophagy Modulator Scoring System (AMSS), which is designed to evaluate methodological integrity. AMSS-A includes the autophagy characterization by 4 aspects, namely, autophagosome quantification, autophagy-related biochemical changes, autophagy substrate degradation, and autophagic flux. AMSS-B contains the pharmacological and functional characteristics of chemical autophagy modulators, including lysosomal function, drug targets, autophagy-dependent pharmacological effects, and validation in multiple cell lines and in vivo models. Our analysis shows that of the 385 studies reporting chemical autophagy modulators, only 142 single studies had examined all 4 aspects of autophagy characterization in AMSS-A, and only 10 out of 142 studies had fulfilled all the AMSS criteria in a single study. A comprehensive analysis of the methodologies used in all the studies was made, along with a summary of studies that demonstrated the highest methodological integrity based on AMSS ranking. To test the reliability of the AMSS, a co-efficiency analysis of scores and co-citation values in the co-citation network was performed, and a significant co-efficiency was obtained. Collectively, AMSS provides insight into the methodological integrity of autophagy modulators studies and also offers a user-friendly toolkit to help choose appropriate assays to characterize autophagy modulators.

KeywordAutophagy Modulator Autophagy Modulator Scoring System Autophagy-monitoring Methods Co-citation Network Methodology
URLView the original
Indexed BySCIE
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000504203600001
Scopus ID2-s2.0-85077167592
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorLu,Jia Hong
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,China
2.Institute of Cancer and Genomic Sciences,College of Medical and Dental Sciences,University of Birmingham,Birmingham,United Kingdom
3.Department of Chemical Biology,Ernest Mario School of Pharmacy,Rutgers University,Piscataway,United States
4.School of Pharmaceutical Sciences,National and Local United Engineering Lab of Druggability and New Drugs Evaluation,Guangdong Provincial Key Laboratory of New Drug Design and Evaluation,Sun Yat-Sen University,Guangzhou,China
5.Key Laboratory of Protein Modification and Degradation,State Key Laboratory of Respiratory Disease,College of Basic Medical Science,Guangzhou Medical University,Guangdong,China
6.Medical College of Acupuncture-Moxibustion and Rehabilitation,Guangzhou University of Chinese Medicine,Guangzhou,China
7.Mr. & Mrs. Ko Chi-Ming Centre for Parkinson’s Disease Research,School of Chinese Medicine,Hong Kong Baptist University,Hong Kong
8.Telethon Institute of Genetics and Medicine (TIGEM),Pozzuoli,Italy
9.Medical Genetics Unit,Department of Medical and Translational Science,Federico II University,Naples,Italy
10.Center for medical genetics,Central South University,Changsha,China
11.Institute of Molecular Precision Medicine,Xiangya Hospital,Central South University,Changsha,China
12.Departments of Neurology and Neuroscience,Friedman Brain Institute,Mount Sinai School of Medicine,New York,United States
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Dong,Yu,Hu,Yuanjia,Sarkar,Sovan,et al. Autophagy modulator scoring system: a user-friendly tool for quantitative analysis of methodological integrity of chemical autophagy modulator studies[J]. Autophagy,2020,16(2):195-202.
APA Dong,Yu,Hu,Yuanjia,Sarkar,Sovan,Zong,Wei Xing,Li,Min,Feng,Du,Song,Ju Xian,Li,Min,Medina,Diego L.,Tan,Jieqiong,Zhang,Zhuohua,Yue,Zhenyu,&Lu,Jia Hong.(2020).Autophagy modulator scoring system: a user-friendly tool for quantitative analysis of methodological integrity of chemical autophagy modulator studies.Autophagy,16(2),195-202.
MLA Dong,Yu,et al."Autophagy modulator scoring system: a user-friendly tool for quantitative analysis of methodological integrity of chemical autophagy modulator studies".Autophagy 16.2(2020):195-202.
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