UM  > Institute of Chinese Medical Sciences
Affiliated with RCfalse
Status已發表Published
Simultaneous determination of α-glucosidase inhibitory triterpenoids in psidium guajava using HPLC-DAD-ELSD and pressurized liquid extraction
Chao,In Cheng1; Chen,Ying1; Gao,Mei Hua2; Lin,Li Gen1; Zhang,Xiao Qi3; Ye,Wen Cai3; Zhang,Qing Wen1
2020-03-11
Source PublicationMolecules
Volume25Issue:6
Abstract

Psidium guajava, a popular food and medicine dual purposes plant cultivated in tropical and subtropical regions, has been widely used as food crop and folk medicine, such as anti-diabetes agent, around the world. Triterpenoids have been considered as the major active ingredients of P. guajava. In the present study, a high-performance liquid chromatography coupled with diode array and evaporative light scattering detectors (HPLC-DAD-ELSD) method was developed for simultaneous determination of nine triterpenoids in P. guajava. Pressurized liquid extraction (PLE) was performed for sample preparation, and the analysis was achieved on a Cosmosil 5C18-MS-II (Nacalai Tesque, Kyoto, Japan) column eluted with gradient 0.1% aqueous formic acid-methanol system. The drift tube temperature of ELSD was set at 40 C, and nitrogen flow-rate was at 1.6 L/min. All calibration curves for the analytes showed good linear regression (R > 0.9992) within test ranges. The established method was validated for intra-day and inter-day precisions (RSDs < 5%) and accuracy (recovery 94.23-106.87%). The validated method was successfully applied to determinate nine triterpenoids in 15 samples from the leave or fruit of P. guajava. In addition, the α-glucosidase inhibition assay showed good α-glucosidase inhibition activity in almost all the determined triterpenoids. The present study suggested that triterpenoids should be the quality control markers for P. guajava and HPLC-DAD-ELSD was an effective tool for the quality control of P. guajava.

KeywordCorosolic Acid Hplc-dad-elsd Pressurized Liquid Extraction Psidium Guajava Triterpenoids Α-glucosidase Inhibitory Effect
DOI10.3390/molecules25061278
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000530248700021
Scopus ID2-s2.0-85081697472
Fulltext Access
Citation statistics
Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorZhang,Xiao Qi; Zhang,Qing Wen
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,999078,Macao
2.Guangdong Institute for Drug Control,Guangzhou,510663,China
3.Institute of Traditional Chinese Medicine and Natural Products,Jinan University,Guangzhou,510632,China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Chao,In Cheng,Chen,Ying,Gao,Mei Hua,et al. Simultaneous determination of α-glucosidase inhibitory triterpenoids in psidium guajava using HPLC-DAD-ELSD and pressurized liquid extraction[J]. Molecules,2020,25(6).
APA Chao,In Cheng,Chen,Ying,Gao,Mei Hua,Lin,Li Gen,Zhang,Xiao Qi,Ye,Wen Cai,&Zhang,Qing Wen.(2020).Simultaneous determination of α-glucosidase inhibitory triterpenoids in psidium guajava using HPLC-DAD-ELSD and pressurized liquid extraction.Molecules,25(6).
MLA Chao,In Cheng,et al."Simultaneous determination of α-glucosidase inhibitory triterpenoids in psidium guajava using HPLC-DAD-ELSD and pressurized liquid extraction".Molecules 25.6(2020).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chao,In Cheng]'s Articles
[Chen,Ying]'s Articles
[Gao,Mei Hua]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chao,In Cheng]'s Articles
[Chen,Ying]'s Articles
[Gao,Mei Hua]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chao,In Cheng]'s Articles
[Chen,Ying]'s Articles
[Gao,Mei Hua]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.