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Affinity-Based Protein Profiling Reveals Cellular Targets of Photoreactive Anticancer Inhibitors
Ma,Nan1; Zhang,Zhi Min1; Lee,Jun Seok3; Cheng,Ke1; Lin,Ligen4; Zhang,Dong Mei1; Hao,Piliang2; Ding,Ke1; Ye,Wen Cai1; Li,Zhengqiu1
2019-11-19
Source PublicationACS Chemical Biology
ISSN1554-8929
Volume14Issue:12Pages:2546-2552
Abstract

Affinity-based protein profiling has proven to be a powerful method in target identification of bioactive molecules. Here, this technology was applied in two photoreactive anticancer inhibitors, arenobufagin and HM30181. Using UV irradiation, these photoreactive reagents can covalently cross-link to target proteins, leading to a covalent binding with target proteins. Moreover, the cellular on/off targets of these two molecules, including ATP1A1, MDR1, PARP1, DDX5, NOP2, RAB6A, and ERGIC1 were first identified by affinity-based protein profiling and bioimaging approaches. The protein hit, PARP1, was further validated to be involved in the function of the anticancer effects.

DOI10.1021/acschembio.9b00784
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000504806100007
Scopus ID2-s2.0-85076375010
Fulltext Access
FWCI1.1829673
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Cited Times [WOS]:17   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorHao,Piliang; Ding,Ke; Ye,Wen Cai; Li,Zhengqiu
Affiliation1.School of Pharmacy,Jinan University,Guangzhou City Key Laboratory of Precision Chemical Drug Development,International Cooperative Laboratory of Traditional Chinese Medicine,Modernization and Innovative Drug Development Ministry of Education of People's Re
2.School of Life Science and Technology,ShanghaiTech University,Shanghai,393 Middle Huaxia Road,201210,China
3.Molecular Recognition Research Center,Korea Institute of Science and Technology (KIST),Seoul,136-791,South Korea
4.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Taipa,999078,Macao
Recommended Citation
GB/T 7714
Ma,Nan,Zhang,Zhi Min,Lee,Jun Seok,et al. Affinity-Based Protein Profiling Reveals Cellular Targets of Photoreactive Anticancer Inhibitors[J]. ACS Chemical Biology,2019,14(12):2546-2552.
APA Ma,Nan,Zhang,Zhi Min,Lee,Jun Seok,Cheng,Ke,Lin,Ligen,Zhang,Dong Mei,Hao,Piliang,Ding,Ke,Ye,Wen Cai,&Li,Zhengqiu.(2019).Affinity-Based Protein Profiling Reveals Cellular Targets of Photoreactive Anticancer Inhibitors.ACS Chemical Biology,14(12),2546-2552.
MLA Ma,Nan,et al."Affinity-Based Protein Profiling Reveals Cellular Targets of Photoreactive Anticancer Inhibitors".ACS Chemical Biology 14.12(2019):2546-2552.
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