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A method to measure the denatured proteins in the corona of nanoparticles based on the specific adsorption of Hsp90ab1
Liu,Shang1; Jiang,Xinbang1; Tian,Xuejiao1; Wang,Zhenzhen1,2; Xing,Zhen1; Chen,Jiahui1; Zhang,Junfeng1; Wang,Chunming2; Dong,Lei1,3
2020-08-07
Source PublicationNanoscale
ISSN2040-3364
Volume12Issue:29Pages:15857-15868
Abstract

The protein corona influences and determines the biological function of nanoparticles (NPs) in vivo. Analysis and understanding of the activities of proteins in coronas are crucial for nanobiology and nanomedicine research. Misfolded proteins in the corona of NPs theoretically exist, and a protein denaturation-related cellular response might occur in this process as well as in related diseases. The exact evaluation of protein denaturation in the corona is valuable to assess the bioactivities of NPs. Here, we found that the level of adsorbed heat shock protein 90 kDa α class B member 1 (Hsp90ab1) by the denatured protein in iron-cobalt-nickel alloy NPs (FeCoNi NPs) and iron oxide NPs (Fe3O4 NPs) was correlated with circular dichroism (CD) analysis and 1-anilinonaphthalene-8-sulfonate (ANS) analysis. The content of Hsp90ab1 in the corona could be easily analysed by western blotting (WB). Further analysis suggested that the method could precisely show the time-dependent protein denaturation on Fe3O4 NPs, as well as the influence of the size and the surface modification. More importantly, this method could be applied to other proteins, like lysozyme, other than albumin. Based on the results and the correlation analysis, incubation and detection of Hsp90ab1 in the NP-corona complex can be used as a new and feasible method to evaluate protein denaturation induced by NPs. 

DOI10.1039/d0nr02297g
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS SubjectChemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
WOS IDWOS:000556582600029
Scopus ID2-s2.0-85089125344
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorDong,Lei
Affiliation1.State Key Laboratory of Pharmaceutical Biotechnology,School of Life Sciences and Medical School,Nanjing University,Nanjing,163 Xianlin Avenue,210093,China
2.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Taipa,Macao
3.Chemistry and Biomedicine Innovative Center,Nanjing University,Nanjing,163 Xianlin Avenue,210093,China
Recommended Citation
GB/T 7714
Liu,Shang,Jiang,Xinbang,Tian,Xuejiao,et al. A method to measure the denatured proteins in the corona of nanoparticles based on the specific adsorption of Hsp90ab1[J]. Nanoscale,2020,12(29):15857-15868.
APA Liu,Shang,Jiang,Xinbang,Tian,Xuejiao,Wang,Zhenzhen,Xing,Zhen,Chen,Jiahui,Zhang,Junfeng,Wang,Chunming,&Dong,Lei.(2020).A method to measure the denatured proteins in the corona of nanoparticles based on the specific adsorption of Hsp90ab1.Nanoscale,12(29),15857-15868.
MLA Liu,Shang,et al."A method to measure the denatured proteins in the corona of nanoparticles based on the specific adsorption of Hsp90ab1".Nanoscale 12.29(2020):15857-15868.
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