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An untargeted metabolomic insight into the high-pressure stress effect on the germination of wholegrain Oryza sativa L.
Ruan,Yifan1; Cai,Zhendong1,2; Deng,Yun3; Pan,Daodong1,2,4; Zhou,Changyu1,2; Cao,Jinxuan1,2; Chen,Xiaojia5; Xia,Qiang1,2,5
2021-02-01
Source PublicationFood Research International
ISSN0963-9969
Volume140
Abstract

High hydrostatic pressure (HHP) technique is used as a novel abiotic stress factor for efficiently enhancing the biosynthesis of selected bioactive phytochemicals in germinated wholegrain, but the information about HHP stress-induced metabolic changes remains rather limited. Thus, the current work employed an untargeted gas chromatography-mass spectrometry-based metabolomic approach combining with multivariate models to analyze the effect of mild HHP stress (30 MPa/5 min) on the overall metabolome shifts of wholegrain brown rice (WBR) during germination. Simultaneously, major phenolics in germinated WBR (GBR) were detected by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry, to explore the potential relationship between HHP stress-induced rice metabolome alternations and the biotransformation of bioactive components. The results demonstrated that the influence of HHP stress on GBR metabolite profiles was defined by germination durations, as revealed by the differentiation of the stressed grains from the naturally germinated grains at different germination points according to principal component analysis. This was further confirmed by the results of orthogonal projections to latent structures discriminant analysis, in which the discriminating metabolites between naturally germinated and HHP-stressed grains varied across the germination process. The metabolite signatures differentiating natural and HHP-stressed germination included glycerol-3-phosphate, monosaccharides, gamma-aminobutyric acid, 2,3-butanediol, glyceryl-glycoside, amino acids and myo-inositol. Besides, HHP stress led to the increase in ribose, arabinitol, salicylic acid, azelaic acid and gamma-aminobutyric acid, as well as the reduced phenolic acids. These results demonstrated that HHP stress before germination matched with appropriate process parameters could be used as a promising technology to tailor metabolic features of germinated products, thus exerting targeted nutrition and health implications.

KeywordBrown Rice High Pressure Stress Nontargeted Metabolomics Opls-da Rice Metabolome Uplc-qtof/ms2 Wholegrain Germination
DOI10.1016/j.foodres.2020.109984
URLView the original
Indexed BySCIE
WOS Research AreaFood Science & Technology
WOS SubjectFood Science & Technology
WOS IDWOS:000623166500002
Scopus ID2-s2.0-85098231369
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Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorXia,Qiang
Affiliation1.Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province,College of Food and Pharmaceutical Sciences,Ningbo University,Ningbo,315832,China
2.State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Ningbo University,Ningbo,315211,China
3.Department of Food Science and Technology,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai,200240,China
4.National R&D Center for Freshwater Fish Processing,Jiangxi Normal University,Nanchang,330022,China
5.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macao
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Ruan,Yifan,Cai,Zhendong,Deng,Yun,et al. An untargeted metabolomic insight into the high-pressure stress effect on the germination of wholegrain Oryza sativa L.[J]. Food Research International,2021,140.
APA Ruan,Yifan,Cai,Zhendong,Deng,Yun,Pan,Daodong,Zhou,Changyu,Cao,Jinxuan,Chen,Xiaojia,&Xia,Qiang.(2021).An untargeted metabolomic insight into the high-pressure stress effect on the germination of wholegrain Oryza sativa L..Food Research International,140.
MLA Ruan,Yifan,et al."An untargeted metabolomic insight into the high-pressure stress effect on the germination of wholegrain Oryza sativa L.".Food Research International 140(2021).
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