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Conditional reprogramming: next generation cell culture
Wu,Xiaoxiao1,2; Wang,Shengpeng3; Li,Mingxing1,2; Li,Jing4; Shen,Jing1,2; Zhao,Yueshui1,2; Pang,Jun5; Wen,Qinglian6; Chen,Meijuan1,2; Wei,Bin7; Kaboli,Parham Jabbarzadeh1,2; Du,Fukuan1,2; Zhao,Qijie1,2; Cho,Chi Hin1,2,8; Wang,Yitao3; Xiao,Zhangang1,2; Wu,Xu1,2
2020-08-01
Source PublicationActa Pharmaceutica Sinica B
ISSN2211-3835
Volume10Issue:8Pages:1360-1381
Abstract

Long-term primary culture of mammalian cells has been always difficult due to unavoidable senescence. Conventional methods for generating immortalized cell lines usually require manipulation of genome which leads to change of important biological and genetic characteristics. Recently, conditional reprogramming (CR) emerges as a novel next generation tool for long-term culture of primary epithelium cells derived from almost all origins without alteration of genetic background of primary cells. CR co-cultures primary cells with inactivated mouse 3T3-J2 fibroblasts in the presence of RHO-related protein kinase (ROCK) inhibitor Y-27632, enabling primary cells to acquire stem-like characteristics while retain their ability to fully differentiate. With only a few years’ development, CR shows broad prospects in applications in varied areas including disease modeling, regenerative medicine, drug evaluation, drug discovery as well as precision medicine. This review is thus to comprehensively summarize and assess current progress in understanding mechanism of CR and its wide applications, highlighting the value of CR in both basic and translational researches and discussing the challenges faced with CR.

Keyword3t3-j2 Fibroblast Conditional Reprogramming Rock Senescence Y-27632
DOI10.1016/j.apsb.2020.01.011
URLView the original
Indexed BySCIE
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000567649600002
Scopus ID2-s2.0-85082507546
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Cited Times [WOS]:17   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorXiao,Zhangang; Wu,Xu
Affiliation1.Laboratory of Molecular Pharmacology,Department of Pharmacology,School of Pharmacy,Southwest Medical University,Luzhou,646000,China
2.South Sichuan Institute of Translational Medicine,Luzhou,646000,China
3.State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Macao
4.Department of Oncology and Hematology,Hospital (T.C.M) Affiliated to Southwest Medical University,Luzhou,646000,China
5.Center of Radiation Oncology,Hospital (T.C.M) Affiliated to Southwest Medical University,Luzhou,646000,China
6.Department of Oncology,the Affiliated Hospital of Southwest Medical University,Southwest Medical University,Luzhou,646000,China
7.College of Pharmaceutical Science & Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals,Zhejiang University of Technology,Hangzhou,310014,China
8.School of Biomedical Sciences,Faculty of Medicine,the Chinese University of Hong Kong,China
Recommended Citation
GB/T 7714
Wu,Xiaoxiao,Wang,Shengpeng,Li,Mingxing,et al. Conditional reprogramming: next generation cell culture[J]. Acta Pharmaceutica Sinica B,2020,10(8):1360-1381.
APA Wu,Xiaoxiao,Wang,Shengpeng,Li,Mingxing,Li,Jing,Shen,Jing,Zhao,Yueshui,Pang,Jun,Wen,Qinglian,Chen,Meijuan,Wei,Bin,Kaboli,Parham Jabbarzadeh,Du,Fukuan,Zhao,Qijie,Cho,Chi Hin,Wang,Yitao,Xiao,Zhangang,&Wu,Xu.(2020).Conditional reprogramming: next generation cell culture.Acta Pharmaceutica Sinica B,10(8),1360-1381.
MLA Wu,Xiaoxiao,et al."Conditional reprogramming: next generation cell culture".Acta Pharmaceutica Sinica B 10.8(2020):1360-1381.
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