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Cross-species recognition of SARS-CoV-2 to bat ACE2
Liu,Kefang1,2,3; Tan,Shuguang1; Niu,Sheng1,4; Wang,Jia5; Wu,Lili1,2,6; Sun,Huan1; Zhang,Yanfang1,7; Pan,Xiaoqian1,2; Qu,Xiao1; Du,Pei6; Meng,Yumin1; Jia,Yunfei1,4; Chen,Qian1,8; Deng,Chuxia3; Yan,Jinghua6,9; Wang,Hong Wei5; Wang,Qihui1,9; Qi,Jianxun1,9; Gao,George Fu1,9
Source PublicationProceedings of the National Academy of Sciences of the United States of America

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has emerged as a major threat to global health. Although varied SARS-CoV-2-related coronaviruses have been isolated from bats and SARS-CoV-2 may infect bat, the structural basis for SARS-CoV-2 to utilize the human receptor counterpart bat angiotensin-converting enzyme 2 (bACE2) for virus infection remains less understood. Here, we report that the SARS-CoV-2 spike protein receptor binding domain (RBD) could bind to bACE2 from Rhinolophus macrotis (bACE2- Rm) with substantially lower affinity compared with that to the human ACE2 (hACE2), and its infectivity to host cells expressing bACE2- Rm was confirmed with pseudotyped SARS-CoV-2 virus and SARS-CoV-2 wild virus. The structure of the SARS-CoV-2 RBD with the bACE2-Rm complex was determined, revealing a binding mode similar to that of hACE2. The analysis of binding details between SARS-CoV-2 RBD and bACE2-Rm revealed that the interacting network involving Y41 and E42 of bACE2-Rm showed substantial differences with that to hACE2. Bats have extensive species diversity and the residues for RBD binding in bACE2 receptor varied substantially among different bat species. Notably, the Y41H mutant, which exists in many bats, attenuates the binding capacity of bACE2-Rm, indicating the central roles of Y41 in the interaction network. These findings would benefit our understanding of the potential infection of SARS-CoV-2 in varied species of bats.

KeywordAce2 Covid-19 Rbd Rhinolophus Macrotis Sars-cov-2
URLView the original
Indexed BySCIE
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000607270100068
Scopus ID2-s2.0-85098135543
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Cited Times [WOS]:35   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorWang,Qihui; Qi,Jianxun; Gao,George Fu
Affiliation1.Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing,100101,China
2.University of Chinese Academy of Sciences,Beijing,100049,China
3.Faculty of Health Sciences,University of Macau,Macau SAR,999078,Macao
4.College of Veterinary Medicine,Shanxi Agricultural University,Jinzhong,030801,China
5.Ministry of Education Key Laboratory of Protein Sciences,Tsinghua-Peking Joint Center for Life Sciences,Beijing Advanced Innovation Center for Structural Biology,Beijing Frontier Research Center of Biological Structures,School of Life Sciences,Tsinghua University,Beijing,100084,China
6.Chinese Academy of Sciences Key Laboratory of Microbial Physiological and Metabolic Engineering,Institute of Microbiology,Chinese Academy of Sciences,Beijing,100101,China
7.Laboratory of Protein Engineering and Vaccines,Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin,300308,China
8.Institute of Physical Science and Information,Anhui University,Hefei,230039,China
9.Savaid Medical School,University of Chinese Academy of Sciences,Beijing,100049,China
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Liu,Kefang,Tan,Shuguang,Niu,Sheng,et al. Cross-species recognition of SARS-CoV-2 to bat ACE2[J]. Proceedings of the National Academy of Sciences of the United States of America,2021,118(1).
APA Liu,Kefang,Tan,Shuguang,Niu,Sheng,Wang,Jia,Wu,Lili,Sun,Huan,Zhang,Yanfang,Pan,Xiaoqian,Qu,Xiao,Du,Pei,Meng,Yumin,Jia,Yunfei,Chen,Qian,Deng,Chuxia,Yan,Jinghua,Wang,Hong Wei,Wang,Qihui,Qi,Jianxun,&Gao,George Fu.(2021).Cross-species recognition of SARS-CoV-2 to bat ACE2.Proceedings of the National Academy of Sciences of the United States of America,118(1).
MLA Liu,Kefang,et al."Cross-species recognition of SARS-CoV-2 to bat ACE2".Proceedings of the National Academy of Sciences of the United States of America 118.1(2021).
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