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Gonadectomy and blood sampling procedures in the small size teleost model japanese medaka (Oryzias latipes)
Royan,Muhammad Rahmad1; Kanda,Shinji2; Kayo,Daichi3; Song,Weiyi4; Ge,Wei4; Weltzien,Finn Arne1; Fontaine,Romain1
2020-12-01
Source PublicationJournal of Visualized Experiments
ISSN1940-087X
Volume2020Issue:166Pages:1-18
Abstract

Sex steroids, produced by the gonads, play an essential role in brain and pituitary tissue plasticity and in the neuroendocrine control of reproduction in all vertebrates by providing feedback to the brain and pituitary. Teleost fishes possess a higher degree of tissue plasticity and variation in reproductive strategies compared to mammals and appear to be useful models to investigate the role of sex steroids and the mechanisms by which they act. The removal of the main source of sex steroid production using gonadectomy together with blood sampling to measure steroid levels has been well-established and fairly feasible in bigger fish and is a powerful technique to investigate the role and effects of sex steroids. However, these techniques raise challenges when implemented in small size teleost models. Here, we describe the step-by-step procedures of gonadectomy in both males and female Japanese medaka followed by blood sampling. These protocols are shown to be highly feasible in medaka indicated by a high survival rate, safety for the life span and phenotype of the fish, and reproducibility in terms of sex steroid clearance. The use of these procedures combined with the other advantages of using this small teleost model will greatly improve the understanding of feedback mechanisms in the neuroendocrine control of reproduction and tissue plasticity provided by sex steroids in vertebrates.

DOI10.3791/62006
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000646166900046
Scopus ID2-s2.0-85097810527
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorFontaine,Romain
Affiliation1.Physiology Unit,Faculty of Veterinary Medicine,Norwegian University of Life Sciences,Norway
2.Laboratory of Physiology,Atmosphere and Ocean Research Institute,The University of Tokyo,Japan
3.Department of Biological Sciences,Graduate School of Science,The University of Tokyo,Japan
4.Centre of Reproduction,Development and Aging (CRDA),Faculty of Health Sciences,University of Macau,Macao
Recommended Citation
GB/T 7714
Royan,Muhammad Rahmad,Kanda,Shinji,Kayo,Daichi,et al. Gonadectomy and blood sampling procedures in the small size teleost model japanese medaka (Oryzias latipes)[J]. Journal of Visualized Experiments,2020,2020(166):1-18.
APA Royan,Muhammad Rahmad,Kanda,Shinji,Kayo,Daichi,Song,Weiyi,Ge,Wei,Weltzien,Finn Arne,&Fontaine,Romain.(2020).Gonadectomy and blood sampling procedures in the small size teleost model japanese medaka (Oryzias latipes).Journal of Visualized Experiments,2020(166),1-18.
MLA Royan,Muhammad Rahmad,et al."Gonadectomy and blood sampling procedures in the small size teleost model japanese medaka (Oryzias latipes)".Journal of Visualized Experiments 2020.166(2020):1-18.
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