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Artemisinin attenuated hydrogen peroxide (H2o2)-induced oxidative injury in SH-SY5Y and hippocampal neurons via the activation of AMPK pathway
Zhao,Xia; Fang,Jiankang; Li,Shuai; Gaur,Uma; Xing,Xingan; Wang,Huan; Zheng,Wenhua
2019-06-01
Source PublicationInternational Journal of Molecular Sciences
ISSN1661-6596
Volume20Issue:11
AbstractOxidative stress is believed to be one of the main causes of neurodegenerative diseases such as Alzheimer’s disease (AD). The pathogenesis of AD is still not elucidated clearly but oxidative stress is one of the key hypotheses. Here, we found that artemisinin, an anti-malarial Chinese medicine, possesses neuroprotective effects. However, the antioxidative effects of artemisinin remain to be explored. In this study, we found that artemisinin rescued SH-SY5Y and hippocampal neuronal cells from hydrogen peroxide (H2O2)-induced cell death at clinically relevant doses in a concentration-dependent manner. Further studies showed that artemisinin significantly restored the nuclear morphology, improved the abnormal changes in intracellular reactive oxygen species (ROS), reduced the mitochondrial membrane potential, and caspase-3 activation, thereby attenuating apoptosis. Artemisinin also stimulated the phosphorylation of the adenosine monophosphate-activated protein kinase (AMPK) pathway in SH-SY5Y cells in a time-and concentration-dependent manner. Inhibition of the AMPK pathway attenuated the protective effect of artemisinin. These data put together suggested that artemisinin has the potential to protect neuronal cells. Similar results were obtained in primary cultured hippocampal neurons. Cumulatively, these results indicated that artemisinin protected neuronal cells from oxidative damage, at least in part through the activation of AMPK. Our findings support the role of artemisinin as a potential therapeutic agent for neurodegenerative diseases.
KeywordAMPK pathway Artemisinin H2O2 Hippocampal neurons SH-SY5Y cells
DOI10.3390/ijms20112680
URLView the original
Language英語English
Scopus ID2-s2.0-85066948772
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Cited Times [WOS]:12   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorZheng,Wenhua
AffiliationCenter of Reproduction,Development & Aging and Institute of Translation Medicine,Faculty of Health Sciences,University of Macau,Taipa,999078,China
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences
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GB/T 7714
Zhao,Xia,Fang,Jiankang,Li,Shuai,et al. Artemisinin attenuated hydrogen peroxide (H2o2)-induced oxidative injury in SH-SY5Y and hippocampal neurons via the activation of AMPK pathway[J]. International Journal of Molecular Sciences,2019,20(11).
APA Zhao,Xia,Fang,Jiankang,Li,Shuai,Gaur,Uma,Xing,Xingan,Wang,Huan,&Zheng,Wenhua.(2019).Artemisinin attenuated hydrogen peroxide (H2o2)-induced oxidative injury in SH-SY5Y and hippocampal neurons via the activation of AMPK pathway.International Journal of Molecular Sciences,20(11).
MLA Zhao,Xia,et al."Artemisinin attenuated hydrogen peroxide (H2o2)-induced oxidative injury in SH-SY5Y and hippocampal neurons via the activation of AMPK pathway".International Journal of Molecular Sciences 20.11(2019).
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