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Integrated approach for confidence-enhanced quantitative analysis of herbal medicines, Cistanche salsa as a case
Liu, Wenjing1; Song, Qingqing1; Yan, Yu1,2; Liu, Yao1; Li, Peng3; Wang, Yitao3; Tu, Pengfei1; Song, Yuelin1; Li, Jun1

Although far away from perfect, it is practical to assess the quality of a given herbal medicine (HM) through simultaneous determination of a panel of components. However, the confidences of the quantitative outcomes from LC-MS/MS platform risk several technical barriers, such as chemical degradation, polarity range, concentration span, and identity misrecognition. Herein, we made an attempt to circumvent these obstacles by integrating several fit-for-purpose techniques, including online extraction (OLE), serially coupled reversed phase LC-hydrophilic interaction liquid chromatography (RPLC-HILIC), tailored multiple reaction monitoring (MRM), and relative response vs. collision energy curve (RRCEC) matching. Confidence-enhanced quantitative analysis of Cistanche salsa (Csa), a well-known psammophytic species and tonic herbal medicine, was conducted as a proof-of-concept. OLE module was deployed to prohibit chemical degradation, in particular E/Z-configuration transformation for phenylethanoid glycosides. Satisfactory retention took place for each analyte regardless of polarity because of successive passing through RPLC and HILIC columns. Optimum parameters for the minor components, at the meanwhile of inferior ones for the abundant ingredients, ensured the locations of all contents in the linear ranges. The unequivocal assignment of the captured signals was achieved by matching retention times, ion transitions, and more importantly, RRCECs between authentic compounds and suspect peaks. Diverse validation assays demonstrated the newly developed method to be reliable. Particularly, the distribution of mannitol rather than galactitol was disclosed although these isomers showed identical retention time and ion transitions. The contents of 21 compounds-of-interest were definitively determined in Csa as well as two analogous species, and the quantitative patterns exerted great variations among not only different species but different Csa samples. Together, the fortification of OLE-RPLC-HILIC-tailored MRM with RRCEC matching could fully address the demands from confidence-enhanced quantitative analysis of HMs. (C) 2018 Elsevier B.V. All rights reserved.

KeywordReversed Phase Liquid Chromatography-hydrophilic Interaction Liquid Chromatography-tailored Multiple Reaction Monitoring Relative Response Vs. Collision Energy Curve Online Extraction Phenylethanoid Glycosides E/z-configuration Transformation
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Indexed BySCIE
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000436225400007
The Source to ArticleWOS
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Cited Times [WOS]:11   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorSong, Yuelin; Li, Jun
Affiliation1.Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
2.Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China
3.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa 999078, Macao
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GB/T 7714
Liu, Wenjing,Song, Qingqing,Yan, Yu,et al. Integrated approach for confidence-enhanced quantitative analysis of herbal medicines, Cistanche salsa as a case[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1561:56-66.
APA Liu, Wenjing,Song, Qingqing,Yan, Yu,Liu, Yao,Li, Peng,Wang, Yitao,Tu, Pengfei,Song, Yuelin,&Li, Jun.(2018).Integrated approach for confidence-enhanced quantitative analysis of herbal medicines, Cistanche salsa as a case.JOURNAL OF CHROMATOGRAPHY A,1561,56-66.
MLA Liu, Wenjing,et al."Integrated approach for confidence-enhanced quantitative analysis of herbal medicines, Cistanche salsa as a case".JOURNAL OF CHROMATOGRAPHY A 1561(2018):56-66.
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