Residential College | false |
Status | 已發表Published |
Automatic range adjustment of the fluorescence immunochromatographic assay based on image processing | |
Jiang,Ruixin1,2; Wu,Huihuang1,2; Yang,Jianpeng1,2; Jiang,Haiyan2; Du,Min1,3; Vai,Mangi2,4![]() ![]() ![]() | |
2020-01 | |
Source Publication | Sensors (Switzerland)
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ISSN | 1424-8220 |
Volume | 20Issue:1 |
Abstract | As an emerging technology, fluorescence immunochromatographic assay (FICA) has the advantages of high sensitivity, strong stability and specificity, which is widely used in the fields of medical testing, food safety and environmental monitoring. The FICA reader based on image processing meets the needs of point-of-care testing because of its simple operation, portability and fast detection speed. However, the image gray level of common image sensors limits the detection range of the FICA reader, and high-precision image sensors are expensive, which is not conducive to the popularization of the instrument. In this paper, FICA strips’ image was collected using a common complementary metal oxide semiconductor (CMOS) image sensor and a range adjustment mechanism was established to automatically adjust the exposure time of the CMOS image sensor to achieve the effect of range expansion. The detection sensitivity showed a onefold increase, and the upper detection limit showed a twofold increase after the proposed method was implemented. In addition, in the experiments of linearity and accuracy, the fitting degree (R) of the fitted curves both reached 0.999. Therefore, the automatic range adjustment method can obviously improve the detection range of the FICA reader based on image processing. |
Keyword | Fluorescence Immunochromatographic Assay Point-of-care Testing Automatic Range Adjustment Image Processing Exposure Time |
DOI | 10.3390/s20010209 |
URL | View the original |
Indexed By | SCIE |
Language | 英語English |
WOS Research Area | Chemistry ; Engineering ; Instruments & Instrumentation |
WOS Subject | Chemistry, Analytical ; Engineering, Electrical & Electronic ; Instruments & Instrumentation |
WOS ID | WOS:000510493100209 |
Scopus ID | 2-s2.0-85077598557 |
Fulltext Access | |
Citation statistics | |
Document Type | Journal article |
Collection | DEPARTMENT OF ELECTRICAL AND COMPUTER ENGINEERING |
Corresponding Author | Gao,Yueming |
Affiliation | 1.Fuzhou University 2.Key Lab of Medical Instrumentation & Pharmaceutical Technology of Fujian Province,Fuzhou,350108,China 3.Key Lab of Eco-Industrial Green Technology of Fujian Province,Nanping,354300,China 4.University of Macau |
Recommended Citation GB/T 7714 | Jiang,Ruixin,Wu,Huihuang,Yang,Jianpeng,et al. Automatic range adjustment of the fluorescence immunochromatographic assay based on image processing[J]. Sensors (Switzerland),2020,20(1). |
APA | Jiang,Ruixin,Wu,Huihuang,Yang,Jianpeng,Jiang,Haiyan,Du,Min,Vai,Mangi,Pun,Siohang,&Gao,Yueming.(2020).Automatic range adjustment of the fluorescence immunochromatographic assay based on image processing.Sensors (Switzerland),20(1). |
MLA | Jiang,Ruixin,et al."Automatic range adjustment of the fluorescence immunochromatographic assay based on image processing".Sensors (Switzerland) 20.1(2020). |
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