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Automatic range adjustment of the fluorescence immunochromatographic assay based on image processing
Jiang,Ruixin1,2; Wu,Huihuang1,2; Yang,Jianpeng1,2; Jiang,Haiyan2; Du,Min1,3; Vai,Mangi2,4; Pun,Siohang4; Gao,Yueming1,2
2020-01
Source PublicationSensors (Switzerland)
ISSN1424-8220
Volume20Issue:1
Abstract

As an emerging technology, fluorescence immunochromatographic assay (FICA) has the advantages of high sensitivity, strong stability and specificity, which is widely used in the fields of medical testing, food safety and environmental monitoring. The FICA reader based on image processing meets the needs of point-of-care testing because of its simple operation, portability and fast detection speed. However, the image gray level of common image sensors limits the detection range of the FICA reader, and high-precision image sensors are expensive, which is not conducive to the popularization of the instrument. In this paper, FICA strips’ image was collected using a common complementary metal oxide semiconductor (CMOS) image sensor and a range adjustment mechanism was established to automatically adjust the exposure time of the CMOS image sensor to achieve the effect of range expansion. The detection sensitivity showed a onefold increase, and the upper detection limit showed a twofold increase after the proposed method was implemented. In addition, in the experiments of linearity and accuracy, the fitting degree (R) of the fitted curves both reached 0.999. Therefore, the automatic range adjustment method can obviously improve the detection range of the FICA reader based on image processing.

KeywordFluorescence Immunochromatographic Assay Point-of-care Testing Automatic Range Adjustment Image Processing Exposure Time
DOI10.3390/s20010209
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaChemistry ; Engineering ; Instruments & Instrumentation
WOS IDWOS:000510493100209
Scopus ID2-s2.0-85077598557
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionDEPARTMENT OF ELECTRICAL AND COMPUTER ENGINEERING
Corresponding AuthorGao,Yueming
Affiliation1.Fuzhou University
2.Key Lab of Medical Instrumentation & Pharmaceutical Technology of Fujian Province,Fuzhou,350108,China
3.Key Lab of Eco-Industrial Green Technology of Fujian Province,Nanping,354300,China
4.University of Macau
Recommended Citation
GB/T 7714
Jiang,Ruixin,Wu,Huihuang,Yang,Jianpeng,et al. Automatic range adjustment of the fluorescence immunochromatographic assay based on image processing[J]. Sensors (Switzerland),2020,20(1).
APA Jiang,Ruixin,Wu,Huihuang,Yang,Jianpeng,Jiang,Haiyan,Du,Min,Vai,Mangi,Pun,Siohang,&Gao,Yueming.(2020).Automatic range adjustment of the fluorescence immunochromatographic assay based on image processing.Sensors (Switzerland),20(1).
MLA Jiang,Ruixin,et al."Automatic range adjustment of the fluorescence immunochromatographic assay based on image processing".Sensors (Switzerland) 20.1(2020).
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