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Deep insights into the green nitrogen removal by anammox in four full-scale WWTPs treating landfill leachate based on 16S rRNA gene and transcripts by 16S rRNA high-throughput sequencing
Yang,Yuchun1,2; Li,Meng3; Hu,Zhong4; Shim,Hojae5; Lin,Jih Gaw6; Li,Xiao Yan7; Gu,Ji Dong8
2020-12-10
Source PublicationJournal of Cleaner Production
ABS Journal Level2
ISSN0959-6526
Volume276Pages:124176
Abstract

Inoculation of anaerobic ammonium oxidation (anammox) bacteria-enriched pellets into the full-scale conventional wastewater treatment plants (WWTPs) treating landfill leachate is an effective and economical way to achieve the engineering application of anammox, a green nitrogen removal process. Current knowledge of the active members in the microbial communities of these WWTPs with active anammox granulation remains limited. In this study, the overall microbial communities in these four WWTPs treating landfill-leachate were analyzed using 16S rRNA gene high-throughput sequencing. At the same time, transcriptionally active groups in these aeration tanks, which were inoculated with exogenous anammox pellets were delineated by the reverse-transcribed 16S rRNA sequencing. Anammox bacteria and nitrifiers were enriched in the aeration tanks benefited from the high ammonium inputs, and the significant accumulation of the slow-growing anammox bacteria was positively related to the biomass immobilization on surfaces of carrier materials. In total, 8610 operational taxonomic units (OTUs) were observed in the four aeration tanks and only 29.0% of all OTUs were transcriptionally active, but the percentage of transcriptionally active members in the 1004 core OTUs (OTUs observed in all aeration tanks) were up to 62.2%. Only 11.6% of all OTUs were the core, but they accounted for up to 67.4% of the total DNA reads. The core microorganisms in the aeration tanks tended to be more abundant and active than the others in the community. Some inactive heterotrophic microbes were also detected with dominance in the microbial communities of the aeration tanks, likely from the dead cells and microbial migrations from influent, suggesting the metabolically functional importance of an organism in the WWTPs cannot be accurately assessed by the relative abundance of taxa based on DNA sequencing. Our findings broaden the current understanding on the active microbial communities of WWTPs for implementing the active anammox process and re-affirm the necessity of differentiating between active and inactive microbial groups in any open ecosystems.

KeywordAnammox Core Organisms Landfill Leachate Microbial Communities Microbial Migration Transcriptionally Active
DOI10.1016/j.jclepro.2020.124176
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaScience & Technology - Other Topics ; Engineering ; Environmental Sciences & Ecology
WOS SubjectGreen & Sustainable Science & Technology ; Engineering, Environmental ; Environmental Sciences
WOS IDWOS:000579500800159
Scopus ID2-s2.0-85091555277
Fulltext Access
FWCI1.5193038
Citation statistics
Document TypeJournal article
CollectionDEPARTMENT OF CIVIL AND ENVIRONMENTAL ENGINEERING
Corresponding AuthorLi,Meng; Gu,Ji Dong
Affiliation1.State Key Laboratory of Biocontrol,School of Ecology,Sun Yat-sen University,Guangzhou,510275,China
2.Laboratory of Environmental Microbiology and Toxicology,School of Biological Sciences,The University of Hong Kong,Hong Kong SAR,Pokfulam Road,Hong Kong
3.Shenzhen Key Laboratory of Marine Microbiome Engineering,Institute for Advanced Study,Shenzhen University,Shenzhen,518060,China
4.Department of Biology,Shantou University,Shantou,515063,China
5.Department of Civil and Environmental Engineering,Faculty of Science and Technology,University of Macau,Macau SAR,China
6.Institute of Environmental Engineering,National Chiao Tung University,Hsinchu City,1001 University Road,30010,Taiwan
7.Department of Civil and Environmental Engineering,The University of Hong Kong,Hong Kong,Pokfulam Road,Hong Kong
8.Environmental Engineering,Guangdong Technion Israel Institute of Technology,Shantou,241 Daxue Road,515063,China
Recommended Citation
GB/T 7714
Yang,Yuchun,Li,Meng,Hu,Zhong,et al. Deep insights into the green nitrogen removal by anammox in four full-scale WWTPs treating landfill leachate based on 16S rRNA gene and transcripts by 16S rRNA high-throughput sequencing[J]. Journal of Cleaner Production,2020,276:124176.
APA Yang,Yuchun,Li,Meng,Hu,Zhong,Shim,Hojae,Lin,Jih Gaw,Li,Xiao Yan,&Gu,Ji Dong.(2020).Deep insights into the green nitrogen removal by anammox in four full-scale WWTPs treating landfill leachate based on 16S rRNA gene and transcripts by 16S rRNA high-throughput sequencing.Journal of Cleaner Production,276,124176.
MLA Yang,Yuchun,et al."Deep insights into the green nitrogen removal by anammox in four full-scale WWTPs treating landfill leachate based on 16S rRNA gene and transcripts by 16S rRNA high-throughput sequencing".Journal of Cleaner Production 276(2020):124176.
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