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Simultaneously quantitative analysis of peptides and chemical components in Cervus and Cucumis polypeptide injection (Songmeile®) using reversed phase liquid chromatography-hydrophilic interaction liquid chromatography–tandem mass spectrometry
Liu, Wenjing1; Cao, Yan1; Ren, Yi1; Xu, Xia1; He, Liqun2; Xia, Ruixue2; Tu, Pengfei1; Wang, Yitao4; Song, Yuelin1,3; Li, Jun1,3
2020-04-26
Source PublicationJournal of Chromatography A
ISSN0021-9673
Volume1617
Abstract

Co-occurrences of peptides and chemical components are usually observed in complicated matrices. Notably, those traditional Chinese medicine prescriptions (TCMPs) contain both plant and animal ingredients. It is still challenging to simultaneously monitor peptides and chemical components attributing to their different liquid chromatographic (LC) and mass spectrometric (MS) behaviors. Herein, efforts were made to pursue an eligible approach enabling simultaneous determination of peptides and chemical components in a TCMP namely Cervus and Cucumis polypeptide injection (CCPI, Songmeile®) that is prepared from the acid hydrolytic peptide-enriched extract of Sika deer (Cervus nippon Temminck) bone and the aqueous extract of muskmelon (Cucumis melo L.) seeds. Reversed phase liquid chromatography and hydrophilic interaction liquid chromatography were serially connected (RPLC-HILIC) to achieve comprehensive retention and separation. Sensitive detection was accomplished with selected reaction monitoring (SRM) mode, and multiply charged and singly charged ion transitions were defined for peptides and chemical components, respectively. Inter-batch variations of CCPI were evaluated in an authentic compound-independent manner. In particular, online energy-resolved MS was proposed to gain optimal parameters for five targeted peptides after that CCPI peptidome was profiled using nanoLC-LTQ Orbitrap Velos Pro MS. A so-called universal metabolome standard (UMS) sample was built for calibration curve construction and subsequently applied to acquire the quasi-contents of all 31 analytes, including five peptides and 26 chemical components, in ten batches of CCPI (CCPI1–CCPI10). The quantitative dataset revealed mild fluctuation for the quasi-content profiles of analytes-of-interest within different batches. More importantly, RPLC-HILIC–SRM is a promising method to fully address the demands of simultaneous measurement of peptides and chemical components in complicated matrices, and it might be a robust analytical tool for in-depth quality evaluation of CCPI as well as other TCMPs.

KeywordCervus And Cucumis Polypeptide Injection Multiply Charged Ion transitIons Online Energy-resolved Mass Spectrometry Peptides Reversed Phase Liquid Chromatography-hydrophilic Interaction Liquid Chromatography
DOI10.1016/j.chroma.2019.460827
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000527316600013
Scopus ID2-s2.0-85077170072
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Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorSong, Yuelin; Li, Jun
Affiliation1.Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 100029, China
2.Harbin Gloria Pharmaceuticals Co. Ltd., Harbin, 150025, China
3.Beijing Key Lab for Quality Evaluation of Chinese Meteria Medica, Beijing University of Chinese Medicine, Beijing, 100029, China
4.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, 999078, Macao
Recommended Citation
GB/T 7714
Liu, Wenjing,Cao, Yan,Ren, Yi,et al. Simultaneously quantitative analysis of peptides and chemical components in Cervus and Cucumis polypeptide injection (Songmeile®) using reversed phase liquid chromatography-hydrophilic interaction liquid chromatography–tandem mass spectrometry[J]. Journal of Chromatography A,2020,1617.
APA Liu, Wenjing,Cao, Yan,Ren, Yi,Xu, Xia,He, Liqun,Xia, Ruixue,Tu, Pengfei,Wang, Yitao,Song, Yuelin,&Li, Jun.(2020).Simultaneously quantitative analysis of peptides and chemical components in Cervus and Cucumis polypeptide injection (Songmeile®) using reversed phase liquid chromatography-hydrophilic interaction liquid chromatography–tandem mass spectrometry.Journal of Chromatography A,1617.
MLA Liu, Wenjing,et al."Simultaneously quantitative analysis of peptides and chemical components in Cervus and Cucumis polypeptide injection (Songmeile®) using reversed phase liquid chromatography-hydrophilic interaction liquid chromatography–tandem mass spectrometry".Journal of Chromatography A 1617(2020).
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