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Accelerating precision anti-cancer therapy by time-lapse and label-free 3D tumor slice culture platform
Xing, Fuqiang1,2,3,6; Liu, Yu Cheng1; Huang, Shigao1; Lyu, Xueying1,2; Su, Sek Man1,2; Chan, Un In1,2; Wu, Pei Chun1; Yan, Yinghan1; Ai, Nana4; Li, Jianjie1,2; Zhao, Ming1,2; Rajendran, Barani Kumar1,2; Liu, Jianlin1,2; Shao, Fangyuan1,2; Sun, Heng1,2,3; Choi, Tak Kan1,2; Zhu, Wenli5; Luo, Guanghui5; Liu, Shuiming5; Xu, De Li5; Chan, Kin Long5; Zhao, Qi1,2,3; Miao, Kai1,2,3; Luo, Kathy Qian1,2,3; Ge, Wei3,4; Xu, Xiaoling1,2,3; Wang, Guanyu6; Liu, Tzu Ming1,2,3; Deng, Chu Xia1,2,3
2021
Source PublicationTheranostics
Volume11Issue:19Pages:9415-9430
Abstract

The feasibility of personalized medicine for cancer treatment is largely hampered by costly, labor-intensive and time-consuming models for drug discovery. Herein, establishing new pre-clinical models to tackle these issues for personalized medicine is urgently demanded. Methods: We established a three-dimensional tumor slice culture (3D-TSC) platform incorporating label-free techniques for time-course experiments to predict anti-cancer drug efficacy and validated the 3D-TSC model by multiphoton fluorescence microscopy, RNA sequence analysis, histochemical and histological analysis. Results: Using time-lapse imaging of the apoptotic reporter sensor C3 (C3), we performed cell-based high-throughput drug screening and shortlisted high-efficacy drugs to screen murine and human 3D-TSCs, which validate effective candidates within 7 days of surgery. Histological and RNA sequence analyses demonstrated that 3D-TSCs accurately preserved immune components of the original tumor, which enables the successful achievement of immune checkpoint blockade assays with antibodies against PD-1 and/or PD-L1. Label-free multiphoton fluorescence imaging revealed that 3D-TSCs exhibit lipofuscin autofluorescence features in the time-course monitoring of drug response and efficacy. Conclusion: This technology accelerates precision anti-cancer therapy by providing a cheap, fast, and easy platform for anti-cancer drug discovery.

Keyword3d Tumor Slice Culture Apoptosis Fret Technique Label-free Personalized Medicine
DOI10.7150/THNO.59533
URLView the original
Language英語English
Scopus ID2-s2.0-85117220524
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionDEPARTMENT OF BIOMEDICAL SCIENCES
Faculty of Health Sciences
Affiliation1.Cancer Center, Faculty of Health Sciences, University of Macau, Macao
2.Centre for Precision Medicine Research and Training, Faculty of health Sciences, University of Macau, Macao
3.MOE Frontier Science Centre for Precision Oncology, University of Macau, Macao
4.Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Macao
5.Kiang Wu Hospital, Macao
6.Department of Biology, School of Life Sciences & Guangdong, Provincial Key Laboratory of Computational Science and Material Design, Southern University of Science and Technology, Shenzhen, China
First Author AffilicationCancer Centre;  Faculty of Health Sciences;  University of Macau
Recommended Citation
GB/T 7714
Xing, Fuqiang,Liu, Yu Cheng,Huang, Shigao,et al. Accelerating precision anti-cancer therapy by time-lapse and label-free 3D tumor slice culture platform[J]. Theranostics,2021,11(19):9415-9430.
APA Xing, Fuqiang.,Liu, Yu Cheng.,Huang, Shigao.,Lyu, Xueying.,Su, Sek Man.,Chan, Un In.,Wu, Pei Chun.,Yan, Yinghan.,Ai, Nana.,Li, Jianjie.,Zhao, Ming.,Rajendran, Barani Kumar.,Liu, Jianlin.,Shao, Fangyuan.,Sun, Heng.,Choi, Tak Kan.,Zhu, Wenli.,Luo, Guanghui.,Liu, Shuiming.,...&Deng, Chu Xia.(2021).Accelerating precision anti-cancer therapy by time-lapse and label-free 3D tumor slice culture platform.Theranostics,11(19),9415-9430.
MLA Xing, Fuqiang,et al."Accelerating precision anti-cancer therapy by time-lapse and label-free 3D tumor slice culture platform".Theranostics 11.19(2021):9415-9430.
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