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Identification and determination of fructooligosaccharides in snow chrysanthemum ( Coreopsis tinctoria nutt.)
Chen, Ling Xiao; Hu, De Jun; Xu, Wen Fei; Li, Shao Ping; Zhao, Jing
2021-01
Source PublicationWorld Journal of Traditional Chinese Medicine
ISSN2311-8571
Volume7Issue:1Pages:78-85
Abstract

Objective: Small molecules in snow chrysanthemum such as flavonoids, phenolic compounds and amino acids have been extensively investigated. No study to date has focused on water-soluble oligosaccharides. The objective of this study is identification and determination of water-soluble oligosaccharides in snow chrysanthemum. Methods: The oligosaccharides in snow chrysanthemum were identified by high performance thin layer chromatography (HPTLC), liquid chromatography-mass spectrometry (LC-MS) combined MS library and methylation analysis for the first time. Subsequently the oligosaccharides were determined by high performance liquid chromatography with a charged aerosol detector (HPLC-CAD). Results: The oligosaccharides in snow chrysanthemum were identified as inulin-type fructooligosaccharides (FOS). The yield of FOS (DP3~DP13) in the first extraction was over 97.6%. The RSDs of repeatability in three sample amount levels (0.08 g, 0.1 g, 0.12 g) are lower than 4.8% and the RSDs of stability are less than 3.5%. The recoveries of FOS (DP3~13) were ranging from 96.9% to 105.6%. The contents of FOS (DP3~DP13) in flowers of snow chrysanthemum from different regions of China were greatly variant. Conclusion: This is the first time to identify and quantify FOS in snow chrysanthemum which is helpful for its performance in the in the fields of biomedical, agriculture and functional food industry as well as development of the quality control methods. In addition, the identification approach developed in this work can also be used for screening potential natural sources containing FOS..

KeywordCoreopsis Tinctoria Nutt Fructooligosaccharides Qaulitative/quantitative Analysis
DOI10.4103/wjtcm.wjtcm_64_20
URLView the original
Indexed ByESCI
Language英語English
WOS Research AreaIntegrative & Complementary Medicine ; Research & Experimental Medicine ; Pharmacology & Pharmacy
WOS SubjectIntegrative & Complementary Medicine ; Medicine, Research & Experimental ; Pharmacology & Pharmacy
WOS IDWOS:000632970400009
Scopus ID2-s2.0-85103256086
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionDEPARTMENT OF PHARMACEUTICAL SCIENCES
Institute of Chinese Medical Sciences
THE STATE KEY LABORATORY OF QUALITY RESEARCH IN CHINESE MEDICINE (UNIVERSITY OF MACAU)
Corresponding AuthorLi, Shao Ping; Zhao, Jing
AffiliationState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Chen, Ling Xiao,Hu, De Jun,Xu, Wen Fei,et al. Identification and determination of fructooligosaccharides in snow chrysanthemum ( Coreopsis tinctoria nutt.)[J]. World Journal of Traditional Chinese Medicine,2021,7(1):78-85.
APA Chen, Ling Xiao,Hu, De Jun,Xu, Wen Fei,Li, Shao Ping,&Zhao, Jing.(2021).Identification and determination of fructooligosaccharides in snow chrysanthemum ( Coreopsis tinctoria nutt.).World Journal of Traditional Chinese Medicine,7(1),78-85.
MLA Chen, Ling Xiao,et al."Identification and determination of fructooligosaccharides in snow chrysanthemum ( Coreopsis tinctoria nutt.)".World Journal of Traditional Chinese Medicine 7.1(2021):78-85.
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