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A dual-functional nanovehicle with fluorescent tracking and its targeted killing effects on hepatocellular carcinoma cells
Zhang, Xiaojie1,2; Gao, Qiming1; Zhuang, Qianfen2; Zhang, Lu3; Wang, Sihan1; Du, Libo2; Yuan, Wenxi4; Wang, Caifang4; Tian, Qiu2; Yu, Hua5; Zhao, Yuming1; Liu, Yang2
2021-03-16
Source PublicationRSC Advances
ISSN2046-2069
Volume11Issue:18Pages:10986-10995
Abstract

All-in-one drug delivery nanovehicles with low cytotoxicity, high clinical imaging tracking capability, and targeted- and controlled-releasing performances are regarded as promising nanoplatforms for tumor theranostics. Recently, the design of these novel nanovehicles by low molecular weight amphiphilic chitosan (CS) was proposed. Based on fluorescent gold nanoclusters (AuNCs), a tumor-targeting nanovehicle (i.e.AuNCs-CS-AS1411) was preparedviaelectrostatic attraction between AuNC-conjugated chitosan (i.e.AuNCs-CS) and the anti-nucleolin aptamer, AS1411. After that, the anticancer drug methotrexate (MTX) was encapsulated into the nanovehicles and then the dual-functional nano-drug (i.e.MTX@AuNCs-CS-AS1411) was comparatively supplied to the human hepatocellular carcinoma cell line HepG2 and the human normal liver cell line LO2, to exhibit its “all in one” behavior. Under the conditions of the same concentration of MTX, MTX@AuNCs-CS-AS1411 demonstrates more intensive cytotoxicity and apoptosis-inducing activity against HepG2 cells than those against normal LO2 cells, mainly due to the targeting effect of AS1411 on the nucleolins that were found at high levels on the surface of tumor cells, but are at low levels or absent on normal cells. On the other hand, the MTX release from the MTX@AuNCs-CS-AS1411 was much faster in mildly acidic solution than that in neutral pH. Thus, it may provide a possibility to more significantly release MTX in intracellular lysosome of tumor cells, rather than let loose MTX during transport of the drug from blood vessels to tumor tissue. In conclusion, our dual-functional nanovehicle possesses high fluorescence efficiency and photostability, low cytotoxicity, pH-dependent controlled release, high sensitivity and target-specificity to cancer cells which allowed concurrent targeted imaging and delivery in cancer chemotherapies.

DOI10.1039/d0ra10486h
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000629770100060
Scopus ID2-s2.0-85102725613
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorZhao, Yuming
Affiliation1.Department of Pharmacology, School of Basic Medical Sciences, Capital Medical University, Beijing, China
2.State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China
3.School of Biomedical Engineering, Capital Medical University, Beijing, China
4.School of Pharmaceutical Sciences, Capital Medical University, Beijing, China
5.Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macao
Recommended Citation
GB/T 7714
Zhang, Xiaojie,Gao, Qiming,Zhuang, Qianfen,et al. A dual-functional nanovehicle with fluorescent tracking and its targeted killing effects on hepatocellular carcinoma cells[J]. RSC Advances,2021,11(18):10986-10995.
APA Zhang, Xiaojie,Gao, Qiming,Zhuang, Qianfen,Zhang, Lu,Wang, Sihan,Du, Libo,Yuan, Wenxi,Wang, Caifang,Tian, Qiu,Yu, Hua,Zhao, Yuming,&Liu, Yang.(2021).A dual-functional nanovehicle with fluorescent tracking and its targeted killing effects on hepatocellular carcinoma cells.RSC Advances,11(18),10986-10995.
MLA Zhang, Xiaojie,et al."A dual-functional nanovehicle with fluorescent tracking and its targeted killing effects on hepatocellular carcinoma cells".RSC Advances 11.18(2021):10986-10995.
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