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Genomics-informed insights into microbial degradation of N,N-dimethylformamide
Li, Junhui1,2; Dijkstra, Paul2,3; Lu, Qihong4; Wang, Shanquan4; Chen, Shaohua5; Li, Deqiang6; Wang, Zhiheng7; Jia, Zhenglei8; Wang, Lu9; Shim, Hojae10
2021-09-01
Source PublicationInternational Biodeterioration and Biodegradation
ISSN0964-8305
Volume163
Abstract

Effective degradation of N,N-Dimethylformamide (DMF), an important industrial waste product, is challenging as only few bacterial isolates are known to degrade DMF. Aerobic remediation has typically been used, whereas anoxic remediation attempts are recently made, using nitrate as one electron acceptor, and ideally include methane as a byproduct. Here, we analyzed 20,762 complete genomes and 28 constructed draft genomes for genes associated with DMF degradation. We identified 952 genomes that harbor genes involved in DMF degradation, expanding the known diversity of prokaryotes with these metabolic capabilities. Our findings suggest plasmids play important roles in DMF degradation in the order Rhizobiales and genus Paracoccus, but not in most other lineages. Degradation pathway analysis reveals that most putative DMF degraders using aerobic Pathway I will accumulate methylamine intermediate, while around 6% of the DMF degraders that are primarily members of Paracoccus, Rhodococcus, Achromobacter, and Pseudomonas could potentially mineralize DMF completely. The aerobic DMF degradation via Pathway II is more common than thought and is primarily present in α-, and β-Proteobacteria and Actinobacteria. Around half (446/952) of putative DMF degraders could grow with nitrate anaerobically (Pathway III), however, genes for the use of methyl-CoM to produce methane were not found. These analyses suggest that microbial consortia could be more advantageous in DMF degradation than pure culture, particularly for methane production under the anaerobic condition. The identified genomes and plasmids form an important foundation for optimizing bioremediation of DMF-containing wastewaters.

KeywordBiodegradation Pathways Denitrification Genomics Methanogenesis Plasmid
DOI10.1016/j.ibiod.2021.105283
URLView the original
Language英語English
WOS Research AreaBiotechnology & Applied Microbiology ; Environmental Sciences & Ecology
WOS SubjectBiotechnology & Applied Microbiology ; Environmental Sciences & Ecology
WOS IDWOS:000676107900005
Scopus ID2-s2.0-85107957804
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorWang, Zhiheng; Jia, Zhenglei; Wang, Lu; Shim, Hojae
Affiliation1.Vanderbilt Microbiome Initiative, Department of Biological Sciences, Vanderbilt University, Nashville, 37235, United States
2.Center for Ecosystem Science and Society, Northern Arizona University, Flagstaff, 86011, United States
3.Department of Biological Sciences, Northern Arizona University, Flagstaff, 86011, United States
4.Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-Sen University, Guangzhou, 510275, China
5.Guangdong Laboratory for Lingnan Modern Agriculture, Integrative Microbiology Research Center, South China Agricultural University, Guangzhou, 510642, China
6.Department of Pharmacy, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, China
7.The Sixth Geological Brigade, Hebei Bureau of Geology and Mineral Resources Exploration, Shijiazhuang, 050000, China
8.Pearl River Water Resources Research Institute, Guangzhou, 510611, China
9.Guangdong Province Key Laboratory for Land Use and Consolidation, South China Agricultural University, Guangzhou, 510642, China
10.Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Macau SAR, 999078, China
Corresponding Author AffilicationFaculty of Science and Technology
Recommended Citation
GB/T 7714
Li, Junhui,Dijkstra, Paul,Lu, Qihong,et al. Genomics-informed insights into microbial degradation of N,N-dimethylformamide[J]. International Biodeterioration and Biodegradation,2021,163.
APA Li, Junhui,Dijkstra, Paul,Lu, Qihong,Wang, Shanquan,Chen, Shaohua,Li, Deqiang,Wang, Zhiheng,Jia, Zhenglei,Wang, Lu,&Shim, Hojae.(2021).Genomics-informed insights into microbial degradation of N,N-dimethylformamide.International Biodeterioration and Biodegradation,163.
MLA Li, Junhui,et al."Genomics-informed insights into microbial degradation of N,N-dimethylformamide".International Biodeterioration and Biodegradation 163(2021).
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