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Lysophosphatidic acid shifts metabolic and transcriptional landscapes to induce a distinct cellular state in human pluripotent stem cells
Xu, Faxiang1; Deng, Chunhao1; Ren, Zhili1; Sun, Liangyu2; Meng, Ya1,3; Liu, Weiwei1,2; Wan, Jianbo4; Chen, Guokai1,5
2021-11-30
Source PublicationCell Reports
Volume37Issue:9
Abstract

Pluripotent stem cells (PSCs) can be maintained in a continuum of cellular states with distinct features. Exogenous lipid supplements can relieve the dependence on de novo lipogenesis and shift global metabolism. However, it is largely unexplored how specific lipid components regulate metabolism and subsequently the pluripotency state. In this study, we report that the metabolic landscape of human PSCs (hPSCs) is shifted by signaling lipid lysophosphatidic acid (LPA), which naturally exists. LPA leads to a distinctive transcriptome profile that is not associated with de novo lipogenesis. Although exogenous lipids such as cholesterol, common free fatty acids, and LPA can affect cellular metabolism, they are not necessary for maintaining primed pluripotency. Instead, LPA induces distinct and reversible phenotypes in cell cycle, morphology, and mitochondria. This study reveals a distinct primed state that could be used to alter cell physiology in hPSCs for basic research and stem cell applications.

KeywordCellular State Hpsc Lipid Lpa Metabolism Mitochondria Pluripotency Transcriptome
DOI10.1016/j.celrep.2021.110063
URLView the original
Language英語English
Scopus ID2-s2.0-85119909030
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Macau, China
2.Bioimaging and Stem Cell Core Facility, Faculty of Health Sciences, University of Macau, Macau, China
3.Center of Interventional Radiology, Zhuhai Precision Medical Center, Zhuhai People's Hospital, Jinan University, Zhuhai, 519000, China
4.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China
5.MoE Frontiers Science Center for Precision Oncology, University of Macau, Macau SAR, China
First Author AffilicationCentre of Reproduction, Development and Aging
Recommended Citation
GB/T 7714
Xu, Faxiang,Deng, Chunhao,Ren, Zhili,et al. Lysophosphatidic acid shifts metabolic and transcriptional landscapes to induce a distinct cellular state in human pluripotent stem cells[J]. Cell Reports,2021,37(9).
APA Xu, Faxiang,Deng, Chunhao,Ren, Zhili,Sun, Liangyu,Meng, Ya,Liu, Weiwei,Wan, Jianbo,&Chen, Guokai.(2021).Lysophosphatidic acid shifts metabolic and transcriptional landscapes to induce a distinct cellular state in human pluripotent stem cells.Cell Reports,37(9).
MLA Xu, Faxiang,et al."Lysophosphatidic acid shifts metabolic and transcriptional landscapes to induce a distinct cellular state in human pluripotent stem cells".Cell Reports 37.9(2021).
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