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Hepatoprotective effect of Qushihuayu formula on non-alcoholic steatohepatitis induced by MCD diet in rat
Lan, Qingping1; Ren, Zhitao1; Chen, Yan1; Cui, Guozhen2; Choi, I. Cheong3; Ung, Carolina Oi Lam1; Yu, Hon Ho3; Lee, Simon Ming Yuen1,4
2021-12-01
Source PublicationChinese Medicine
Volume16Issue:1
Abstract

Background: Non-alcoholic steatohepatitis (NASH) is an advanced form of non-alcoholic fatty liver disease (NAFLD) for which there is yet any standard pharmacotherapy. Traditional Chinese medicine formula such as Qushihuayu (QSHY) composing of multiple bioactive compounds has been used to treat NAFLD and NASH and shows beneficial effects over single compound treatment. This study aimed to investigate the mechanism of hepatoprotective effect of QSHY formula using a rat model. Methods: Six-weeks old male Wistar rats were given methionine/choline supplemented (MCS) diet for 8 weeks and used as the blank control. Another 7 rats, which received methionine/choline deficient (MCD) diet in the first 6 weeks and a MCS&MCD (1:1) mixture diet in the last 2 weeks, were used as the model group. The groups of QSHY pre-treatment, low dosage, medium dosage and high dosage were given the same diet as the model group. Except for pre-treatment group (1 week in advanced of other groups), all QSHY treatment groups received QSHY formula by gavage every day since the MCD diet started. Results: In the MCD diet group, the QSHY formula decreased the serum ALT and AST levels, lipid droplets, inflammation foci, FAS and α-SMA protein expression than MCD diet group. MAPK pathways phospharylation were markedly depressed by the QSHY formula. Moreover, QSHY formula enhanced PPAR-γ and p-p65 translocating into nucleus. The administration of QSHY increased hepatic mRNA levels of Transcription Factor 1 alpha (HNF1A), Hepatocyte Nuclear Factor 4 alpha (HNF4A) and Forkhead box protein A3 (FOXA3) which play a pivotal role in Hepatic stellate cell (HSCs) reprogramming. Conclusion: These findings suggest that QSHY formula exerts a hepatoprotective effect against steatosis and fibrosis presumably via depressed MAPK pathways phosphorylation, reinforcement of PPAR-γ and p-p65 translocating into nucleus and enhanced HSCs reprogramming.

KeywordFibrosis Hscs Reprogramming Inflammation Mapk Pathway Methionine-choline Deficient Diet Non-alcoholic Fatty Liver Disease Non-alcoholic Steatohepatitis P-p65 Ppar-γ Qushihuayu Traditional Chinese Medicine
DOI10.1186/s13020-021-00434-1
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS SubjectIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000629588500001
Scopus ID2-s2.0-85102599633
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Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionDEPARTMENT OF PHARMACEUTICAL SCIENCES
Institute of Chinese Medical Sciences
THE STATE KEY LABORATORY OF QUALITY RESEARCH IN CHINESE MEDICINE (UNIVERSITY OF MACAU)
Corresponding AuthorYu, Hon Ho; Lee, Simon Ming Yuen
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao
2.Zun Yi Medical University- Zhuhai Campus, Zhuhai, China
3.Department of Gastroenterology, Kiang Wu Hospital, Macao
4.Institute of Chinese Medical Sciences, University of Macau, Taipa, Room 7003, N22 Building, Avenide da Universidade, Macao
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Lan, Qingping,Ren, Zhitao,Chen, Yan,et al. Hepatoprotective effect of Qushihuayu formula on non-alcoholic steatohepatitis induced by MCD diet in rat[J]. Chinese Medicine,2021,16(1).
APA Lan, Qingping,Ren, Zhitao,Chen, Yan,Cui, Guozhen,Choi, I. Cheong,Ung, Carolina Oi Lam,Yu, Hon Ho,&Lee, Simon Ming Yuen.(2021).Hepatoprotective effect of Qushihuayu formula on non-alcoholic steatohepatitis induced by MCD diet in rat.Chinese Medicine,16(1).
MLA Lan, Qingping,et al."Hepatoprotective effect of Qushihuayu formula on non-alcoholic steatohepatitis induced by MCD diet in rat".Chinese Medicine 16.1(2021).
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