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The first Conus genome assembly reveals a primary genetic central dogma of conopeptides in C. betulinus
Peng, Chao1,2; Huang, Yu1,3; Bian, Chao1,3,4; Li, Jia1; Liu, Jie5,6; Zhang, Kai1,3,10; You, Xinxin1,3; Lin, Zhilong5; He, Yanbin5; Chen, Jieming1,3; Lv, Yunyun1,3,11; Ruan, Zhiqiang1,3; Zhang, Xinhui1; Yi, Yunhai1,3; Li, Yanping1,11; Lin, Xueqiang1; Gu, Ruobo1; Xu, Junmin1; Yang, Jia’an7; Fan, Chongxu8; Yao, Ge8; Chen, Ji Sheng8; Jiang, Hui8; Gao, Bingmiao2; Shi, Qiong1,3,9
2021-12-01
Source PublicationCell Discovery
ISSN2056-5968
Volume7Issue:1
Abstract

Although there are various Conus species with publicly available transcriptome and proteome data, no genome assembly has been reported yet. Here, using Chinese tubular cone snail (C. betulinus) as a representative, we sequenced and assembled the first Conus genome with original identification of 133 genome-widely distributed conopeptide genes. After integration of our genomics, transcriptomics, and peptidomics data in the same species, we established a primary genetic central dogma of diverse conopeptides, assuming a rough number ratio of ~1:1:1:10s for the total genes: transcripts: proteins: post-translationally modified peptides. This ratio may be special for this worm-hunting Conus species, due to the high diversity of various Conus genomes and the big number ranges of conopeptide genes, transcripts, and peptides in previous reports of diverse Conus species. Only a fraction (45.9%) of the identified conotopeptide genes from our achieved genome assembly are transcribed with transcriptomic evidence, and few genes individually correspond to multiple transcripts possibly due to intraspecies or mutation-based variances. Variable peptide processing at the proteomic level, generating a big diversity of venom conopeptides with alternative cleavage sites, post-translational modifications, and N-/C-terminal truncations, may explain how the 133 genes and ~123 transcripts can generate thousands of conopeptides in the venom of individual C. betulinus. We also predicted many conopeptides with high stereostructural similarities to the putative analgesic ω-MVIIA, addiction therapy AuIB and insecticide ImI, suggesting that our current genome assembly for C. betulinus is a valuable genetic resource for high-throughput prediction and development of potential pharmaceuticals.

DOI10.1038/s41421-021-00244-7
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000620594400001
Scopus ID2-s2.0-85101209338
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorGao, Bingmiao; Shi, Qiong
Affiliation1.Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen, 518083, China
2.Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory of Research and Development of Herbs, School of Pharmacy, Hainan Medical University, Haikou, 570102, China
3.BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, 518083, China
4.Center of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, 999078, Macao
5.BGI-Shenzhen, BGI, Shenzhen, 518083, China
6.China National GeneBank, BGI, Shenzhen, 518120, China
7.Micro Pharmtech Ltd., Wuhan, 430000, China
8.Research Institute of Pharmaceutical Chemistry, Beijing, 102205, China
9.Laboratory of Fish Genomics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518061, China
10.College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China
11.College of Life Sciences, Neijiang Normal University, Neijiang, 641100, China
Recommended Citation
GB/T 7714
Peng, Chao,Huang, Yu,Bian, Chao,et al. The first Conus genome assembly reveals a primary genetic central dogma of conopeptides in C. betulinus[J]. Cell Discovery,2021,7(1).
APA Peng, Chao.,Huang, Yu.,Bian, Chao.,Li, Jia.,Liu, Jie.,Zhang, Kai.,You, Xinxin.,Lin, Zhilong.,He, Yanbin.,Chen, Jieming.,Lv, Yunyun.,Ruan, Zhiqiang.,Zhang, Xinhui.,Yi, Yunhai.,Li, Yanping.,Lin, Xueqiang.,Gu, Ruobo.,Xu, Junmin.,Yang, Jia’an.,...&Shi, Qiong.(2021).The first Conus genome assembly reveals a primary genetic central dogma of conopeptides in C. betulinus.Cell Discovery,7(1).
MLA Peng, Chao,et al."The first Conus genome assembly reveals a primary genetic central dogma of conopeptides in C. betulinus".Cell Discovery 7.1(2021).
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