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B-Myb Mediates Proliferation and Migration of Non-Small-Cell Lung Cancer via Suppressing IGFBP3
Fan, Xiaoyan1,2,3; Wang, Yitao1,2; Jiang, Tinghui1,2; Cai, Wei1,2; Jin, Yuelei1,2,4; Niu, Yulong2; Zhu, Huifang1,2; Bu, Youquan1,2
2018-05
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1422-0067
Volume19Issue:5
AbstractB-Myb has been shown to play an important oncogenic role in several types of human cancers, including non-small-cell lung cancer (NSCLC). We previously found that B-Myb is aberrantly upregulated in NSCLC, and overexpression of B-Myb can significantly promote NSCLC cell growth and motility. In the present study, we have further investigated the therapeutic potential of B-Myb in NSCLC. Kaplan-Meier and Cox proportional hazards analysis indicated that high expression of B-Myb is significantly associated with poor prognosis in NSCLC patients. A loss-of-function study demonstrated that depletion of B-Myb resulted in significant inhibition of cell growth and delayed cell cycle progression in NSCLC cells. Notably, B-Myb depletion also decreased NSCLC cell migration and invasion ability as well as colony-forming ability. Moreover, an in vivo study demonstrated that B-Myb depletion caused significant inhibition of tumor growth in a NSCLC xenograft nude mouse model. A molecular mechanistic study by RNA-seq analysis revealed that B-Myb depletion led to deregulation of various downstream genes, including insulin-like growth factor binding protein 3 (IGFBP3). Overexpression of IGFBP3 suppressed the B-Myb-induced proliferation and migration, whereas knockdown of IGFBP3 significantly rescued the inhibited cell proliferation and motility caused by B-Myb siRNA (small interfering RNA). Expression and luciferase reporter assays revealed that B-Myb could directly suppress the expression of IGFBP3. Taken together, our results suggest that B-Myb functions as a tumor-promoting gene via suppressing IGFBP3 and could serve as a novel therapeutic target in NSCLC.
KeywordB-Myb IGFBP3 NSCLC proliferation motility
DOI10.3390/ijms19051479
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000435297000215
PublisherMDPI
The Source to ArticleWOS
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Cited Times [WOS]:18   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.ChongQing Med Univ, Dept Biochem & Mol Biol, Coll Basic Med Sci, Chongqing 400016, Peoples R China;
2.Chongqing Med Univ, Mol Med & Canc Res Ctr, Chongqing 400016, Peoples R China;
3.Jiamusi Univ, Dept Pathol, Coll Basic Med Sci, Jiamusi 154007, Peoples R China;
4.Jiamusi Univ, Dept Cell Biol, Coll Basic Med Sci, Jiamusi 154007, Peoples R China
Recommended Citation
GB/T 7714
Fan, Xiaoyan,Wang, Yitao,Jiang, Tinghui,et al. B-Myb Mediates Proliferation and Migration of Non-Small-Cell Lung Cancer via Suppressing IGFBP3[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,19(5).
APA Fan, Xiaoyan,Wang, Yitao,Jiang, Tinghui,Cai, Wei,Jin, Yuelei,Niu, Yulong,Zhu, Huifang,&Bu, Youquan.(2018).B-Myb Mediates Proliferation and Migration of Non-Small-Cell Lung Cancer via Suppressing IGFBP3.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(5).
MLA Fan, Xiaoyan,et al."B-Myb Mediates Proliferation and Migration of Non-Small-Cell Lung Cancer via Suppressing IGFBP3".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.5(2018).
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