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Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography
Li, SP1,2; Li, P1; Lai, CM1; Gong, YX1; Kan, KKW1; Dong, TTX3; Tsim, KWK3; Wang, YT1
2004-05-21
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1036Issue:2Pages:239-243
Abstract

A simple method is described for the simultaneous determination of ergosterol, nucleosides and their bases in Cordyceps. The samples were extracted byusing pressurised liquid extraction (PLE). The effects of experimental variables, such as solvent, temperature, static extraction time and cycles, on PLE efficiency have been studied. The results showed a strong influence of the solvent and temperature on extraction efficiency of PLE. The determination was achieved by high-performance liquid chromatography (HPLC) using a Zorbax NH2 analytical column (250 x 4.6 mm i.d., 5 mum) with diode-array detector (DAD). The automated preparation of the sample permits a very fast analysis which is an important goal for routine purpose.

KeywordCordyceps Spp Pharmaceutical Analysis Ergosterol Sterols Nucleosides
DOI10.1016/j.chroma.2004.02.080
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000221103200020
Scopus ID2-s2.0-1842635382
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Cited Times [WOS]:103   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionDEPARTMENT OF PHARMACEUTICAL SCIENCES
Institute of Chinese Medical Sciences
Corresponding AuthorLi, SP
Affiliation1.Inst. of Chinese Medical Sciences, University of Macau, Taipa, Macau, Macao
2.Natl. Std. Laboraory Traditional C., Nanjing Univ. Traditional Chinese M., Nanjing, China
3.Department of Biology, Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, Hong Kong
First Author AffilicationUniversity of Macau
Corresponding Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Li, SP,Li, P,Lai, CM,et al. Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2004,1036(2):239-243.
APA Li, SP,Li, P,Lai, CM,Gong, YX,Kan, KKW,Dong, TTX,Tsim, KWK,&Wang, YT.(2004).Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1036(2),239-243.
MLA Li, SP,et al."Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1036.2(2004):239-243.
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