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Simultaneously enantiospecific determination of (+)-trans-khellactone, (+/-)-praeruptorin A, (+/-)-praeruptorin B, (+)-praeruptorin E, and their metabolites, (+/-)-cis-khellactone, in rat plasma using
Song, Yuelin1; Jing, Wanghui1; Yang, Fengqing2; Shi, Zhan3; Yao, Meicun3; Yan, Ru1; Wang, Yitao1

Many chiral drugs are used as the racemic mixtures in clinical practice. The occurrence of enantioselectively pharmacological activities calls for the development of enantiospecific analytical approaches during pharmacokinetic studies of enantiomers. Sample preparation plays a key role during quantitative analysis of biological samples. In current study, a rapid and reliable online solid phase extraction-chiral high performance liquid chromatography-tandem mass spectrometry (online SPE-chiral LC-MS/MS) method was developed for the simultaneously enantiospecific quantitation of(+)-trans-khellactone (dTK), (+/-)-cis-khellactone (d/lCK), (+/-)-praeruptorin A (d/lPA), (+/-)-praeruptorin B (d/lPB) and (+)praeruptorin E (dPE), the main active angular-type pyranocoumarins (APs) in Peucedani Radix (Chinese name: Qian-hu) or the major metabolites of those APs, in rat plasma. The validation assay results described here show good selectivity and enantiospecificity, extraction efficiency, accuracy and precision with quantification limits (LOQs) of2.57, 1.28, 1.28, 1.88, 4.16, 4.16 and 4.18 ng mL(-1) for dTK, lCK, dCK, dPA, dPB, lPB and dPE, respectively, while IPA was not detected in rat plasma due to the carboxylesterase(s)mediated hydrolysis. In addition, the validated system was satisfactorily applied to characterize the pharmacokinetic properties of those components in normal and chronic obstructive pulmonary disease (COPD) rats following oral administration of Qian-hu extract. dCK and lCK were observed as the main herb-related compounds in plasma. Enantioselectively pharmacokinetic profiles occurred for dCK vs lCK, dPA vs IPA, and dPB vs lPB in either normal or COPD rats. The proposed whole system is expected to be a preferable analytical tool for in vivo study of chiral drugs, in particular for the characterization of enantioselectively pharmacokinetic profiles. (C) 2013 Elsevier B.V. All rights reserved.

KeywordOnline Spe Chiral Lc-ms/ms Enantiospecific Peucedani Radix Pharmacokinetics
Indexed BySCIE
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000329532800037
The Source to ArticleWOS
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Cited Times [WOS]:30   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Taipa 999078, Peoples R China
2.Chongqing Univ, Sch Chem & Chem Engn, Chongqing 401331, Peoples R China
3.Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China
First Author AffilicationUniversity of Macau
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GB/T 7714
Song, Yuelin,Jing, Wanghui,Yang, Fengqing,et al. Simultaneously enantiospecific determination of (+)-trans-khellactone, (+/-)-praeruptorin A, (+/-)-praeruptorin B, (+)-praeruptorin E, and their metabolites, (+/-)-cis-khellactone, in rat plasma using[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2014,88(1):269-277.
APA Song, Yuelin,Jing, Wanghui,Yang, Fengqing,Shi, Zhan,Yao, Meicun,Yan, Ru,&Wang, Yitao.(2014).Simultaneously enantiospecific determination of (+)-trans-khellactone, (+/-)-praeruptorin A, (+/-)-praeruptorin B, (+)-praeruptorin E, and their metabolites, (+/-)-cis-khellactone, in rat plasma using.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,88(1),269-277.
MLA Song, Yuelin,et al."Simultaneously enantiospecific determination of (+)-trans-khellactone, (+/-)-praeruptorin A, (+/-)-praeruptorin B, (+)-praeruptorin E, and their metabolites, (+/-)-cis-khellactone, in rat plasma using".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 88.1(2014):269-277.
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