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Comprehensive quantitative analysis of Shuang-Huang-Lian oral liquid using UHPLC-Q-TOF-MS and HPLC-ELSD
Tian-Bo Zhang1; Rui-Qi Yue1; Jun Xu1; Hing-Man Ho1; Dik-Lung Ma2; Chung-Hang Leung3; Siu-Leung Chau1; Zhong-Zhen Zhao1; Hu-Biao Chen1; Quan-Bin Han1
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN1873264X 07317085

Shuang-Huang-Lian oral liquid (SHL) is a well-known Chinese patent drug containing three herbal medicines: Radix Scutellariae, Flos Lonicerae Japonicae and Fructus Forsythiae. It is usually used to treat acute upper respiratory tract infection caused by virus or bacteria. Although the licensing of botanical drug Veregen approved by FDA has indicated the importance of quantitative analysis in quality control of herbal medicines, quantitative evaluation of a Chinese patent drug like SHL remains a challenge due to the complex chemical profile. In this study, 15 small molecular components of SHL (four flavonoids, six quinic acid derivatives, three saponins and two phenylethanoid glycosides) were simultaneously determined using ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). The contents of the three major saccharides, namely fructose, glucose and sucrose were quantified using high performance liquid chromatography-evaporative light scattering detector on an amino column (HPLC-ELSD). The macromolecules were quantified by precipitating in 80% ethanol, drying the precipitate, and then weighing. The established methods were validated in terms of linearity, sensitivity, precision, accuracy and stability and then successfully applied to analyze 12 batches of commercial products of SHL produced by four different manufacturers. The results indicated that 57.52-78.11% (w/w) of SHL could be quantitatively determined (non-saccharide small molecules: 1.77-3.75%, monosaccharides: 0.93-20.93%, macromolecules: 2.63-5.76% and sucrose: 49.20-65.94%). This study may provide a useful way to comprehensively evaluate the quality of SHL.

KeywordChinese Patent Drug Quality Evaluation Quantitative Analysis Saccharides Determination Shuang-huang-lian Oral Liquid
URLView the original
Indexed BySCIE
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000347277500001
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Cited Times [WOS]:16   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorHu-Biao Chen; Quan-Bin Han
Affiliation1.School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China
2.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
3.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
Recommended Citation
GB/T 7714
Tian-Bo Zhang,Rui-Qi Yue,Jun Xu,et al. Comprehensive quantitative analysis of Shuang-Huang-Lian oral liquid using UHPLC-Q-TOF-MS and HPLC-ELSD[J]. Journal of Pharmaceutical and Biomedical Analysis,2015,102:1-8.
APA Tian-Bo Zhang,Rui-Qi Yue,Jun Xu,Hing-Man Ho,Dik-Lung Ma,Chung-Hang Leung,Siu-Leung Chau,Zhong-Zhen Zhao,Hu-Biao Chen,&Quan-Bin Han.(2015).Comprehensive quantitative analysis of Shuang-Huang-Lian oral liquid using UHPLC-Q-TOF-MS and HPLC-ELSD.Journal of Pharmaceutical and Biomedical Analysis,102,1-8.
MLA Tian-Bo Zhang,et al."Comprehensive quantitative analysis of Shuang-Huang-Lian oral liquid using UHPLC-Q-TOF-MS and HPLC-ELSD".Journal of Pharmaceutical and Biomedical Analysis 102(2015):1-8.
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