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Progestin increases the expression of gonadotropins in pituitaries of male zebrafish
Wang, Cuili1,2; Liu, Dongteng1,2; Chen, Weiting3; Ge, Wei3; Hong, Wanshu1,2; Zhu, Yong1,4; Chen, Shi X.1,2,5

Our previous study showed that the in vivo positive effects of 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (DHP), the major progestin in zebrafish, on early spermatogenesis was much stronger than the ex vivo ones, which may suggest an effect of DHP on the expression of gonadotropins. In our present study, we first observed that fshb and lhb mRNA levels in the pituitary of male adult zebrafish were greatly inhibited by 3 weeks exposure to 10 nM estradiol (E-2). However, an additional 24 h 100 nM DHP exposure not only reversed the E-2-induced inhibition, but also significantly increased the expression of fshb and lhb mRNA. These stimulatory effects were also observed in male adult fish without E-2 pretreatment, and a time course experiment showed that it took 24 h for fshb and 12 h for lhb to respond significantly. Because these stimulatory activities were partially antagonized by a nuclear progesterone receptor (Pgr) antagonist mifepristone, we generated a Pgr-knockout (pgr(-/-)) model using the TALEN technique. With and without DHP in vivo treatment, fshb and lhb mRNA levels of pgr(-/-) were significantly lower than those of pgr(+/+). Furthermore, ex vivo treatment of pituitary fragments of pgr(-/-) with DHP stimulated lhb, but not fshb mRNA expression. Results from double-colored fluorescent in situ hybridization showed that pgr mRNA was expressed only in fshb-expressing cells. Taken together, our results indicated that DHP participated in the regulation of neuroendocrine control of reproduction in male zebrafish, and exerted a Pgr-mediated direct stimulatory effect on fshb mRNA at pituitary level.

Keyword17 Alpha,20 Beta-dihydroxy-4-pregnen-3-one Gonadotropin Nuclear Progesterone Receptor Zebrafish Pituitary
URLView the original
Indexed BySCIE
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000379880200016
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Cited Times [WOS]:13   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China
2.Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, China
3.Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences, University of Macau, Taipa, Macau, China
4.Department of Biology, East Carolina University, Greenville, North Carolina, USA
5.State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen, China
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GB/T 7714
Wang, Cuili,Liu, Dongteng,Chen, Weiting,et al. Progestin increases the expression of gonadotropins in pituitaries of male zebrafish[J]. JOURNAL OF ENDOCRINOLOGY,2016,230(1):143-156.
APA Wang, Cuili,Liu, Dongteng,Chen, Weiting,Ge, Wei,Hong, Wanshu,Zhu, Yong,&Chen, Shi X..(2016).Progestin increases the expression of gonadotropins in pituitaries of male zebrafish.JOURNAL OF ENDOCRINOLOGY,230(1),143-156.
MLA Wang, Cuili,et al."Progestin increases the expression of gonadotropins in pituitaries of male zebrafish".JOURNAL OF ENDOCRINOLOGY 230.1(2016):143-156.
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