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Tak1, Smad4 and Trim33 redundantly mediate TGF-β3 signaling during palate development
Lane J.5; Yumoto K.5; Azhar M.3; Ninomiya-Tsuji J.4; Inagaki M.4; Hu Y.1; Deng C.-X.6; Kim J.2; Mishina Y.5; Kaartinen V.5
Source PublicationDevelopmental Biology
ISSN1095564X 00121606

Transforming growth factor-beta3 (TGF-β3) plays a critical role in palatal epithelial cells by inducing palatal epithelial fusion, failure of which results in cleft palate, one of the most common birth defects in humans. Recent studies have shown that Smad-dependent and Smad-independent pathways work redundantly to transduce TGF-β3 signaling in palatal epithelial cells. However, detailed mechanisms by which this signaling is mediated still remain to be elucidated. Here we show that TGF-β activated kinase-1 (Tak1) and Smad4 interact genetically in palatal epithelial fusion. While simultaneous abrogation of both Tak1 and Smad4 in palatal epithelial cells resulted in characteristic defects in the anterior and posterior secondary palate, these phenotypes were less severe than those seen in the corresponding Tgfb3 mutants. Moreover, our results demonstrate that Trim33, a novel chromatin reader and regulator of TGF-β signaling, cooperates with Smad4 during palatogenesis. Unlike the epithelium-specific Smad4 mutants, epithelium-specific Tak1:Smad4- and Trim33:Smad4-double mutants display reduced expression of Mmp13 in palatal medial edge epithelial cells, suggesting that both of these redundant mechanisms are required for appropriate TGF-β signal transduction. Moreover, we show that inactivation of Tak1 in Trim33:Smad4 double conditional knockouts leads to the palatal phenotypes which are identical to those seen in epithelium-specific Tgfb3 mutants. To conclude, our data reveal added complexity in TGF-β signaling during palatogenesis and demonstrate that functionally redundant pathways involving Smad4, Tak1 and Trim33 regulate palatal epithelial fusion.

KeywordPalatogenesis Smad4 Tak1 Tgf-β3 Signaling Trim33
URLView the original
Indexed BySCIE
WOS Research AreaDevelopmental Biology
WOS SubjectDevelopmental Biology
WOS IDWOS:000348975000009
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Cited Times [WOS]:32   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorKaartinen V.
Affiliation1.Frederick National Laboratory for Cancer Research
2.Children's Hospital Los Angeles
3.Indiana University-Purdue University Indianapolis
4.North Carolina State University
5.University of Michigan School of Dentistry
6.Universidade de Macau
Recommended Citation
GB/T 7714
Lane J.,Yumoto K.,Azhar M.,et al. Tak1, Smad4 and Trim33 redundantly mediate TGF-β3 signaling during palate development[J]. Developmental Biology,2015,398(2):231-241.
APA Lane J.,Yumoto K.,Azhar M.,Ninomiya-Tsuji J.,Inagaki M.,Hu Y.,Deng C.-X.,Kim J.,Mishina Y.,&Kaartinen V..(2015).Tak1, Smad4 and Trim33 redundantly mediate TGF-β3 signaling during palate development.Developmental Biology,398(2),231-241.
MLA Lane J.,et al."Tak1, Smad4 and Trim33 redundantly mediate TGF-β3 signaling during palate development".Developmental Biology 398.2(2015):231-241.
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