UM  > Faculty of Health Sciences
Affiliated with RCfalse
Serum proteomic fingerprints of adult patients with severe acute respiratory syndrome
Pang R.T.K.; Poon T.C.W.; Chan K.C.A.; Lee N.L.S.; Chiu R.W.K.; Tong Y.-K.; Wong R.M.Y.; Chim S.S.C.; Ngai S.M.; Sung J.J.Y.; Lo Y.M.D.
Source PublicationClinical Chemistry
AbstractBackground: Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by a new coronavirus strain, SARS-CoV. Specific proteomic patterns might be present in serum in response to the infection and could be useful for early detection of the disease. Methods: Using surface-enhanced laser desorption/ionization (SELDI) ProteinChip technology, we profiled and compared serum proteins of 39 patients with early-stage SARS infection and 39 non-SARS patients who were suspected cases during the SARS outbreak period. Proteomic patterns associated with SARS were identified by bioinformatic and biostatistical analyses. Features of interest were then purified and identified by tandem mass spectrometry. Results: Twenty proteomic features were significantly different between the 2 groups. Fifteen were increased in the SARS group, and 5 were decreased. Their concentrations were correlated with 2 or more clinical and/or biochemical variables. Two were correlated with the SARS-CoV viral load. Hierarchical clustering analysis showed that a majority of the SARS patients (95%) had similar serum proteomic profiles and identified 2 subgroups with poor prognosis. ROC curve analysis identified individual features as potential biomarkers for SARS diagnosis (areas under ROC curves, 0.733-0.995). ROC curve areas were largest for an N-terminal fragment of complement C3c α chain (m/z 28 119) and an internal fragment of fibrinogen α-E chain (m/z 5908). Immunoglobulin κ light chain (m/z 24 505) positively correlated with viral load. Conclusions: Specific proteomic fingerprints in the sera of adult SARS patients could be used to identify SARS cases early during onset with high specificity and sensitivity. © 2006 American Association for Clinical Chemistry.
URLView the original
Fulltext Access
Citation statistics
Cited Times [WOS]:65   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationPrince of Wales Hospital Hong Kong
Recommended Citation
GB/T 7714
Pang R.T.K.,Poon T.C.W.,Chan K.C.A.,et al. Serum proteomic fingerprints of adult patients with severe acute respiratory syndrome[J]. Clinical Chemistry,2006,52(3):421-429.
APA Pang R.T.K.,Poon T.C.W.,Chan K.C.A.,Lee N.L.S.,Chiu R.W.K.,Tong Y.-K.,Wong R.M.Y.,Chim S.S.C.,Ngai S.M.,Sung J.J.Y.,&Lo Y.M.D..(2006).Serum proteomic fingerprints of adult patients with severe acute respiratory syndrome.Clinical Chemistry,52(3),421-429.
MLA Pang R.T.K.,et al."Serum proteomic fingerprints of adult patients with severe acute respiratory syndrome".Clinical Chemistry 52.3(2006):421-429.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Pang R.T.K.]'s Articles
[Poon T.C.W.]'s Articles
[Chan K.C.A.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Pang R.T.K.]'s Articles
[Poon T.C.W.]'s Articles
[Chan K.C.A.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Pang R.T.K.]'s Articles
[Poon T.C.W.]'s Articles
[Chan K.C.A.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.