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SDS-PAGE and 2-DE protein profiles of Ganoderma lucidum from different origins
Li, Chun-Hong1; Zuo, Hua-Li1; Chen, Cen1; Hu, Yuan-Jia2; Qian, Zheng-Ming3; Li, Wen-Jia3; Xia, Zhi-Ning1; Yang, Feng-Qing1

Ganoderma lucidum (Chizhi in Chinese) is one of the most valuable and widely used medicinal fungi in traditional Chinese medicines (TCMs). Most of previous studies were focused on the triterpenoids and polysaccharides of G lucidum, whereas less attention had been paid on the protein, which is another bioactive compound in it. In the present study, protein maps of fourteen G lucidum samples were comprehensively analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2-DE). The results indicated that there were significant differences in protein profiles of G lucidum samples from different origins. Furthermore, previous reported bioactive proteins from the fruiting bodies of G lucidum, were mainly distributed in 4 taxa (A, B, C and D) based on their molecular weights on the 2-DE maps. The proteins should be considered as marker for the quality control of G lucidum, because the proteomic variation may affect on their pharmacological activities.

KeywordGanoderma Lucidum Sds-page 2-de Bioactive Protein Protein Profiles
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Indexed BySCIE
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000428228300013
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYang, Feng-Qing
Affiliation1.School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China
3.Sunshine Lake Pharma Co., Ltd., Guangdong, China
Recommended Citation
GB/T 7714
Li, Chun-Hong,Zuo, Hua-Li,Chen, Cen,et al. SDS-PAGE and 2-DE protein profiles of Ganoderma lucidum from different origins[J]. PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES,2018,31(2):447-454.
APA Li, Chun-Hong,Zuo, Hua-Li,Chen, Cen,Hu, Yuan-Jia,Qian, Zheng-Ming,Li, Wen-Jia,Xia, Zhi-Ning,&Yang, Feng-Qing.(2018).SDS-PAGE and 2-DE protein profiles of Ganoderma lucidum from different origins.PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES,31(2),447-454.
MLA Li, Chun-Hong,et al."SDS-PAGE and 2-DE protein profiles of Ganoderma lucidum from different origins".PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES 31.2(2018):447-454.
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