| Status | 已發表Published |
| Standardized median difference for quality control in high-throughput screening | |
Huang W.2; Zhang X.D.1 ; Li Y.2; Wang W.W.1; Soper K.1
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| 2012-10-26 | |
| Source Publication | Proceedings of 2012 International Symposium on Information Technologies in Medicine and Education, ITME 2012 |
| Volume | 1 |
| Pages | 515-518 |
| Abstract | High-throughput screening (HTS) of large-scale ribonucleic acid interference (RNAi) libraries has been widely used in functional genomics in recent years. The Z-factor and strictly standardized mean difference (SSMD) have already become two popular methods for quality control (QC) in HTS experiments. This paper is intended to put forward a robust parameter, standardized median difference (SMD), for group comparison and derive some statistical properties of SMD, such as maximum likelihood estimate (MLE), moment-method (MM) estimate and plug-in estimate of SMD as well as their relative asymptotical distributions. Using the simulations, the plug-in estimate of SMD obtains QC results robust to outliers whereas the Z-factor and SSMD obtain QC results sensitive to outliers. Meanwhile, compared to other QC metrics, the SMD also obtains non-inferior QC results when there are no outliers. © 2012 IEEE. |
| DOI | 10.1109/ITiME.2012.6291354 |
| URL | View the original |
| Language | 英語English |
| Fulltext Access | |
| Citation statistics | |
| Document Type | Conference paper |
| Collection | Faculty of Health Sciences |
| Affiliation | 1.Merck Research Laboratories 2.Beijing Normal University |
| Recommended Citation GB/T 7714 | Huang W.,Zhang X.D.,Li Y.,et al. Standardized median difference for quality control in high-throughput screening[C],2012:515-518. |
| APA | Huang W.,Zhang X.D.,Li Y.,Wang W.W.,&Soper K..(2012).Standardized median difference for quality control in high-throughput screening.Proceedings of 2012 International Symposium on Information Technologies in Medicine and Education, ITME 2012,1,515-518. |
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