UM  > Faculty of Health Sciences
Affiliated with RCfalse
Status已發表Published
Genome-wide differential expression profiling of lncRNAs and mRNAs in human induced pluripotent stem cell-derived endothelial cells exposed to e-cigarette extract
Le, Hoai Huong Thi1; Liu, Chen wei1; Denaro, Philip1; Jousma, Jordan2; Shao, Ning Yi3; Rahman, Irfan4; Lee, Won Hee1
2021-12-01
Source PublicationStem Cell Research and Therapy
ISSN1757-6512
Volume12Issue:1
Abstract

Background: Electronic-cigarette (e-cig) usage, particularly in the youth population, is a growing concern. It is known that e-cig causes endothelial dysfunction, which is a risk factor for the development of cardiovascular diseases; however, the mechanisms involved remain unclear. We hypothesized that long noncoding RNAs (lncRNAs) may play a role in e-cig-induced endothelial dysfunction. Methods: Here, we identified lncRNAs that are dysregulated in human induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) following 24 h of e-cig aerosol extract treatment via microarray analysis. We performed Gene Ontology and Kyoto Encyclopedia of Genes and Genome (KEGG) pathway analyses of the dysregulated mRNAs following e-cig exposure and constructed co-expression networks of the top 5 upregulated lncRNAs and the top 5 downregulated lncRNAs and the mRNAs that are correlated with them. Furthermore, the functional effects of knocking down lncRNA lung cancer-associated transcript 1 (LUCAT1) on EC phenotypes were determined as it was one of the significantly upregulated lncRNAs following e-cig exposure based on our profiling. Results: 183 lncRNAs and 132 mRNAs were found to be upregulated, whereas 297 lncRNAs and 413 mRNAs were found to be downregulated after e-cig exposure. We also observed that e-cig caused dysregulation of endothelial metabolism resulting in increased FAO activity, higher mitochondrial membrane potential, and decreased glucose uptake and glycolysis. These results suggest that e-cig alters EC metabolism by increasing FAO to compensate for energy deficiency in ECs. Finally, the knockdown of LUCAT1 prevented e-cig-induced EC dysfunction by maintaining vascular barrier, reducing reactive oxygen species level, and increasing migration capacity. Conclusion: This study identifies an expression profile of differentially expressed lncRNAs and several potential regulators and pathways in ECs exposed to e-cig, which provide insights into the regulation of lncRNAs and mRNAs and the role of lncRNA and mRNA networks in ECs associated e-cig exposure.

KeywordE-cigarettes Endothelial Dysfunction Fatty Acid Oxidation Ipsc-ecs Lncrnas
DOI10.1186/s13287-021-02654-6
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaCell Biology ; Research & Experimental Medicine
WOS SubjectCell & Tissue Engineering ; Cell Biology ; Medicine, Research & Experimental
WOS IDWOS:000726323100007
Scopus ID2-s2.0-85120737092
Fulltext Access
Citation statistics
Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorLee, Won Hee
Affiliation1.Department of Basic Medical Sciences, University of Arizona College of Medicine, Phoenix, 425 N 5th Street, Building ABC1, Rm 426, 85004-2157, United States
2.Department of Pharmacology and Regenerative Medicine, University of Illinois College of Medicine, Chicago, 60612, United States
3.Health Sciences, University of Macau, Macao
4.Department of Environmental Medicine, University of Rochester Medical Center, Rochester, 14642, United States
Recommended Citation
GB/T 7714
Le, Hoai Huong Thi,Liu, Chen wei,Denaro, Philip,et al. Genome-wide differential expression profiling of lncRNAs and mRNAs in human induced pluripotent stem cell-derived endothelial cells exposed to e-cigarette extract[J]. Stem Cell Research and Therapy,2021,12(1).
APA Le, Hoai Huong Thi,Liu, Chen wei,Denaro, Philip,Jousma, Jordan,Shao, Ning Yi,Rahman, Irfan,&Lee, Won Hee.(2021).Genome-wide differential expression profiling of lncRNAs and mRNAs in human induced pluripotent stem cell-derived endothelial cells exposed to e-cigarette extract.Stem Cell Research and Therapy,12(1).
MLA Le, Hoai Huong Thi,et al."Genome-wide differential expression profiling of lncRNAs and mRNAs in human induced pluripotent stem cell-derived endothelial cells exposed to e-cigarette extract".Stem Cell Research and Therapy 12.1(2021).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Le, Hoai Huong Thi]'s Articles
[Liu, Chen wei]'s Articles
[Denaro, Philip]'s Articles
Baidu academic
Similar articles in Baidu academic
[Le, Hoai Huong Thi]'s Articles
[Liu, Chen wei]'s Articles
[Denaro, Philip]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Le, Hoai Huong Thi]'s Articles
[Liu, Chen wei]'s Articles
[Denaro, Philip]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.