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A silkworm infection model to investigate Vibrio vulnificus virulence genes
Yamamoto M.1; Kashimoto T.2; Yoshimura Y.1; Tachibana N.1; Kuroda S.1; Miki Y.1; Kitabayashi S.1; Tong P.1,3; Xiao J.1,4; Tanaka K.1; Hamamoto H.5; Sekimizu K.5; Yamamoto K.1
Source PublicationMolecular Medicine Reports
ISSN17913004 17912997

The halophilic marine bacterium, Vibrio vulnificus, occasionally causes fatal septicemia in immunocompromised patients. Mice are commonly used as experimental animals to investigate the virulence of V. vulnificus, however, a large number of mice are generally required for bioassays. The present study examined whether the invertebrate species, silkworms, can be used instead of mice to investigate V. vulnificus virulence. When the silkworms were inoculated with 1.2x107 colony forming units of V. vulnificus OPU1 Rf, a virulent strain of V. vulnificus, all injected silkworms died within 48 h, however, those injected with culture filtrate or diluent did not. This silkworm infection model was then used to isolate attenuated V. vulnificus mutants from 1,016 transposon inserted mutants. Consequently, a harmless mutant, SW998, was isolated. In this strain, the transposon was inserted into the rtxA gene, which is a known V. vulnificus virulence gene. In conclusion, the present study demonstrated that silkworms are useful animals for investigating the virulence of V. vulnificus.

KeywordAnimal Model Screening Silkworm Vibrio Vulnificus Virulence
URLView the original
Indexed BySCIE
WOS Research AreaOncology ; Research & Experimental Medicine
WOS SubjectOncology ; Medicine, Research & Experimental
WOS IDWOS:000387241600029
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Okayama Prefectural University
2.Kitasato University
3.Nanchang University
4.University of Tokyo
5.Universidade de Macau
Recommended Citation
GB/T 7714
Yamamoto M.,Kashimoto T.,Yoshimura Y.,et al. A silkworm infection model to investigate Vibrio vulnificus virulence genes[J]. Molecular Medicine Reports,2016,14(5):4243-4247.
APA Yamamoto M.,Kashimoto T.,Yoshimura Y.,Tachibana N.,Kuroda S.,Miki Y.,Kitabayashi S.,Tong P.,Xiao J.,Tanaka K.,Hamamoto H.,Sekimizu K.,&Yamamoto K..(2016).A silkworm infection model to investigate Vibrio vulnificus virulence genes.Molecular Medicine Reports,14(5),4243-4247.
MLA Yamamoto M.,et al."A silkworm infection model to investigate Vibrio vulnificus virulence genes".Molecular Medicine Reports 14.5(2016):4243-4247.
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