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Aptamer-functionalized Ti3C2-MXene Nanosheets with One-step Potentiometric Detection of Programmed Death-ligand 1
Li, Bin1; Pu, Wenyuan1; Weng, Lingyan2; Lyu, Peng4; Xu, Houxi1; Zhang, Wen1; Ge, Lilin1; Kwok, Hang Fai3; Wu, Qinan1
2022-01
Source PublicationElectroanalysis
ISSN1040-0397
Volume34Issue:1Pages:2-7
Abstract

This communication describes a simple sensitive one-step potentiometric aptasensing method for quantitative detection of a referenced therapeutic biomarker (programmed death-ligand 1, PD−L1). The aptasensor is constructed by modifying PD−L1-specific aptamer on TiC-MXene nanosheets-functionalized electrode. Introduction of PD−L1 induces the specific reaction between PD−L1 and aptamer, thereby resulting in the change of spatial structures. The surface electric potential of modified electrode is shifted upon addition of PD−L1 proteins before and after the reaction of aptamer with the analyte. Interestingly, potentiometric aptamer with TiC-MXene nanosheets can achieve a higher sensitivity and a lower detection limit toward target PD−L1 relative to aptamer-modified electrode. Experimental results indicated that the linear range and detection limit of using TiC-MXene nanosheets were 0.01–100 ng mL and 7.8 pg mL PD−L1, respectively. Meanwhile, the specificity, reproducibility, storing stability and accuracy of potentiometric aptasensor are acceptable for the screening of PD−L1 in human serum samples.

DOI10.1002/elan.202100438
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaChemistry ; Electrochemistry
WOS SubjectChemistry, Analytical ; Electrochemistry
WOS IDWOS:000711725800001
Scopus ID2-s2.0-85117935243
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorZhang, Wen; Ge, Lilin
Affiliation1.Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.Nanjing Institute of Tourism & Hospitality, Nanjing, 210023, China
3.Faculty of Health Sciences, University of Macau, Taipa, Avenida de Universidade, 999078, Macao
4.College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian, 350108, China
Recommended Citation
GB/T 7714
Li, Bin,Pu, Wenyuan,Weng, Lingyan,et al. Aptamer-functionalized Ti3C2-MXene Nanosheets with One-step Potentiometric Detection of Programmed Death-ligand 1[J]. Electroanalysis,2022,34(1):2-7.
APA Li, Bin,Pu, Wenyuan,Weng, Lingyan,Lyu, Peng,Xu, Houxi,Zhang, Wen,Ge, Lilin,Kwok, Hang Fai,&Wu, Qinan.(2022).Aptamer-functionalized Ti3C2-MXene Nanosheets with One-step Potentiometric Detection of Programmed Death-ligand 1.Electroanalysis,34(1),2-7.
MLA Li, Bin,et al."Aptamer-functionalized Ti3C2-MXene Nanosheets with One-step Potentiometric Detection of Programmed Death-ligand 1".Electroanalysis 34.1(2022):2-7.
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