UM
Affiliated with RCfalse
Status已發表Published
Targeting PLA2G16, a lipid metabolism gene, by Ginsenoside Compound K to suppress the malignant progression of colorectal cancer
Yang, Li1,2,3,4,5; Zheng, Lingjie1,3,4,5; Xie, Xiaonv1,3,4,5; Luo, Junjia1,3,4,5; Yu, Jing1,3,4,5; Zhang, Lihua1,3,4,5; Meng, Wenhui1,3,4,5; Zhou, Yingen1,3,4,5; Chen, Ling6; Ouyang, Dongsheng1,3,4,5; Zhou, Honghao1,3,4,5; Tan, Zhirong1,3,4,5
2022-02-01
Source PublicationJournal of Advanced Research
ISSN2090-1232
Volume36Pages:265-276
Abstract

Introduction: Colorectal cancer (CRC) is a common malignant tumor with a high global incidence, metastasis rate and low cure rate. Changes in lipid metabolism-related genes can affect the occurrence and development of CRC, and may be a potential therapeutic target for CRC. Therefore, starting from lipid metabolism-related genes to find natural medicines for tumor treatment may become a new direction in CRC research. Objectives: This study aimed to investigate the effect of PLA2G16, a key gene involved in lipid metabolism, on the biological function of CRC, and whether the anti-CRC effect of GCK is related to PLA2G16. Methods: To explore the role of PLA2G16 in CRC in vitro and in vivo, we performed cell proliferation, migration, invasion and nude mice tumorigenesis assays. As for the mechanism, we designed RNA-seq analysis and verified by western blotting and immunofluorescence experiments. Subsequently, we found the anti-CRC effect of GCK is related to PLA2G16 through western blotting and rescue experiments. Results: We showed that PLA2G16 was significantly higher in CRC tissues than the adjacent normal appearing tissues, and high PLA2G16 expression correlates with unfavorable prognosis of CRC patients. Further, PLA2G16 promoted the malignant progression of CRC by inhibiting the Hippo signaling pathway determined by RNA-seq analysis, and GCK exerted anti-CRC effects by inhibiting the protein expression of PLA2G16 in vitro and in vivo. Conclusion: Our results suggested that PLA2G16 promote the malignant progression of CRC by inhibiting the Hippo signaling pathway and the anti-CRC effect of GCK is through inhibiting the protein expression of PLA2G16.

KeywordColorectal Cancer Ginsenoside Compound k Hippo Signaling Pathway Lipid Metabolism Gene Lipidomics Pla2g16
DOI10.1016/j.jare.2021.06.009
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000747012700020
Scopus ID2-s2.0-85108530150
Fulltext Access
Citation statistics
Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorTan, Zhirong
Affiliation1.Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, 410008, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, Avenida da Universidade, China
3.Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha, 410078, China
4.Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, 110 Xiangya Road, 410078, China
5.National Clinical Research Center for Geriatric Disorders, Changsha, 87 Xiangya Road, 410008, China
6.Department of General Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Yang, Li,Zheng, Lingjie,Xie, Xiaonv,et al. Targeting PLA2G16, a lipid metabolism gene, by Ginsenoside Compound K to suppress the malignant progression of colorectal cancer[J]. Journal of Advanced Research,2022,36:265-276.
APA Yang, Li,Zheng, Lingjie,Xie, Xiaonv,Luo, Junjia,Yu, Jing,Zhang, Lihua,Meng, Wenhui,Zhou, Yingen,Chen, Ling,Ouyang, Dongsheng,Zhou, Honghao,&Tan, Zhirong.(2022).Targeting PLA2G16, a lipid metabolism gene, by Ginsenoside Compound K to suppress the malignant progression of colorectal cancer.Journal of Advanced Research,36,265-276.
MLA Yang, Li,et al."Targeting PLA2G16, a lipid metabolism gene, by Ginsenoside Compound K to suppress the malignant progression of colorectal cancer".Journal of Advanced Research 36(2022):265-276.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang, Li]'s Articles
[Zheng, Lingjie]'s Articles
[Xie, Xiaonv]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang, Li]'s Articles
[Zheng, Lingjie]'s Articles
[Xie, Xiaonv]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang, Li]'s Articles
[Zheng, Lingjie]'s Articles
[Xie, Xiaonv]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.