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Role of endocrine disruption in toxicity of 6-benzylaminopurine (6-BA) to early-life stages of Zebrafish
Gong, Guiyi1,2; Kam, Hiotong2; Chen, Hanbin2; Chen, Yan2; Cheang, Wai san2; Giesy, John P.3,4,5; Zhou, Qiaohong6; Lee, Simon Ming yuen2
2022-03-01
Source PublicationEcotoxicology and Environmental Safety
ISSN0147-6513
Volume232
Abstract

6-benzylaminopurine (6-BA), classified as a “plant hormone”, is an important ingredient in production of “toxic bean sprouts”. Although there is no direct evidence of adverse effects, its hazardous effects have received some attention and aroused furious debate between proponents and environmental regulators. In this study, potential adverse effects of 6-BA were investigated by exposing zebrafish in vivo to 0.2 – 25 mg 6-BA/L. Results indicated that, when exposure was limited to early-life stage (4–36 hpf), 20 mg 6-BA/L caused early hatching, abnormal spontaneous movement, and precocious hyperactivity in zebrafish embryos/larvae. While under a continuous exposure regime, 6-BA at 0.2 mg/L was able to cause hyperactive locomotion and transcription of genes related to neurogenesis (gnrh3 and nestin) and endocrine systems (cyp19a and fshb) in 5 dpf larvae. Quantification by use of LC/MS indicated bioaccumulation of 6-BA in zebrafish increased when exposed to 0.2 or 20 mg 6-BA/L. These results suggested that 6-BA could accumulate in aquatic organisms and disrupt neuro-endocrine systems. Accordingly, exposure to 0.2 mg 6-BA/L increased production of estradiol (E2) and consequently E2/T ratio in zebrafish larvae, which directly indicated 6-BA is estrogenic. In silico simulations demonstrated potential for binding of 6-BA to estrogen receptor alpha (ERa) and cytochrome P450 aromatase (CYP19A). Therefore, induction of estrogenic effects, via potential interactions with hormone receptors or disturbance of downstream transcription signaling, was possible mechanism underlying the toxicity of 6-BA. Taken together, these findings demonstrate endocrine disrupting properties of 6-BA, which suggest concerns about risks posed to endocrine systems.

KeywordCyp19a Endocrine Disruptors Estrogenic Neurogenesis Plant Growth Regulator
DOI10.1016/j.ecoenv.2022.113287
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000810924800010
Scopus ID2-s2.0-85124166781
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionDEPARTMENT OF PHARMACEUTICAL SCIENCES
Institute of Chinese Medical Sciences
THE STATE KEY LABORATORY OF QUALITY RESEARCH IN CHINESE MEDICINE (UNIVERSITY OF MACAU)
Corresponding AuthorLee, Simon Ming yuen
Affiliation1.Zhanjiang Institute of Clinical Medicine, Central People's Hospital of Zhanjiang, Zhanjiang, 524045, China
2.State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, China
3.Toxicology Centre, University of Saskatchewan, Saskatoon, S7N 5B3, Canada
4.Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, S7N 5B4, Canada
5.Department of Environmental Sciences, Baylor University, Waco, 76706, United States
6.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Gong, Guiyi,Kam, Hiotong,Chen, Hanbin,et al. Role of endocrine disruption in toxicity of 6-benzylaminopurine (6-BA) to early-life stages of Zebrafish[J]. Ecotoxicology and Environmental Safety,2022,232.
APA Gong, Guiyi,Kam, Hiotong,Chen, Hanbin,Chen, Yan,Cheang, Wai san,Giesy, John P.,Zhou, Qiaohong,&Lee, Simon Ming yuen.(2022).Role of endocrine disruption in toxicity of 6-benzylaminopurine (6-BA) to early-life stages of Zebrafish.Ecotoxicology and Environmental Safety,232.
MLA Gong, Guiyi,et al."Role of endocrine disruption in toxicity of 6-benzylaminopurine (6-BA) to early-life stages of Zebrafish".Ecotoxicology and Environmental Safety 232(2022).
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