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Genome and Transcriptome Sequencing of the Astaxanthin-Producing Green Microalga, Haematococcus pluvialis
Luo, Qiulan1,2; Bian, Chao3,4; Tao, Ming1,2; Huang, Yu3,5; Zheng, Yihong1,6; Lv, Yunyun3,5; Li, Jia3; Wang, Chaogang1; You, Xinxin3; Jia, Bin1,6; Xu, Junmin3; Li, Jiancheng1,6; Li, Ze6; Shi, Qiong1,3,5; Hu, Zhangli1,6
2019-01
Source PublicationGenome Biology and Evolution
ISSN1759-6653
Volume11Issue:1Pages:166-173
Abstract

Haematococcus pluvialis is a freshwater species of Chlorophyta, family Haematococcaceae. It is well known for its capacity to synthesize high amounts of astaxanthin, which is a strong antioxidant that has been utilized in aquaculture and cosmetics. To improve astaxanthin yield and to establish genetic resources for H. pluvialis, we performed whole-genome sequencing, assembly, and annotation of this green microalga. A total of 83.1 Gb of raw reads were sequenced. After filtering the raw reads, we subsequently generated a draft assembly with a genome size of 669.0 Mb, a scaffold N50 of 288.6 kb, and predicted 18,545 genes. We also established a robust phylogenetic tree from 14 representative algae species. With additional transcriptome data, we revealed some novel potential genes that are involved in the synthesis, accumulation, and regulation of astaxanthin production. In addition, we generated an isoform-level reference transcriptome set of 18,483 transcripts with high confidence. Alternative splicing analysis demonstrated that intron retention is the most frequent mode. In summary, we report the first draft genome of H. pluvialis. These genomic resources along with transcriptomic data provide a solid foundation for the discovery of the genetic basis for theoretical and commercial astaxanthin enrichment.

KeywordAnnotation Assembly Astaxanthin Genome Sequencing Haematococcus Pluvialis
DOI10.1093/gbe/evy263
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaEvolutionary Biology ; Genetics & Heredity
WOS SubjectEvolutionary Biology ; Genetics & Heredity
WOS IDWOS:000462712200012
Scopus ID2-s2.0-85060056203
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Cited Times [WOS]:29   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorShi, Qiong; Hu, Zhangli
Affiliation1.Guangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Key Laboratory of Plant Epigenetic, Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Sciences, College of Life Sciences and Oceanography, Shenzhen University
2.Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong, China
3.Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab. of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen, Guangdong, China
4.Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Taipa, China
5.BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, Guangdong, China
6.Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong, China
Recommended Citation
GB/T 7714
Luo, Qiulan,Bian, Chao,Tao, Ming,et al. Genome and Transcriptome Sequencing of the Astaxanthin-Producing Green Microalga, Haematococcus pluvialis[J]. Genome Biology and Evolution,2019,11(1):166-173.
APA Luo, Qiulan,Bian, Chao,Tao, Ming,Huang, Yu,Zheng, Yihong,Lv, Yunyun,Li, Jia,Wang, Chaogang,You, Xinxin,Jia, Bin,Xu, Junmin,Li, Jiancheng,Li, Ze,Shi, Qiong,&Hu, Zhangli.(2019).Genome and Transcriptome Sequencing of the Astaxanthin-Producing Green Microalga, Haematococcus pluvialis.Genome Biology and Evolution,11(1),166-173.
MLA Luo, Qiulan,et al."Genome and Transcriptome Sequencing of the Astaxanthin-Producing Green Microalga, Haematococcus pluvialis".Genome Biology and Evolution 11.1(2019):166-173.
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