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A quantitative method for polysaccharides based on endo-enzymatic released specific oligosaccharides: A case of Lentinus edodes
Deng, Yong1,2; Chen, Ling Xiao1,2; Zhu, Bao Jie1,2; Zhao, Jing1,2; Li, Shao Ping1,2
2022-04-30
Source PublicationInternational Journal of Biological Macromolecules
ISSN0141-8130
Volume205Pages:15-22
Abstract

Polysaccharides exhibit multiple pharmacological activities, which are closely related to their structural characteristics. Therefore, quantitative quality control of polysaccharides based on chemical properties is of importance for their applications. However, polysaccharides are mixed macromolecular compounds that are difficult to separate, and the lack of standards made direct quantification more difficult. In this study, we proposed a new quantitative method based on the released specific oligosaccharides for polysaccharides from Lentinus edodes (shiitake) and other related fungi. Specific oligosaccharides were firstly released from polysaccharides using 1,3-β-glucanase, then derivatized with 2-aminobenzamide (2-AB), which further separated by hydrophilic interaction chromatography (HILIC) and quantitatively determined by UPLC coupled with fluorescence detector (FLR). Laminaritriose was used as the universal standard for quantification of all the oligosaccharides. This method was validated according to linearity, limit of detection, limit of quantitation, precision, accuracy, repeatability and stability. In addition, the four specific oligosaccharides released from polysaccharides in L. edodes were qualitatively analyzed by extracted ion chromatogram (EIC) from UPLC-MS profiles, which were identified to be disaccharide, trisaccharide and tetrasccharide. The proposed strategy not only realized the quantitative analysis of polysaccharides by UPLC-FLR, but also could achieve the qualitative distinction of different polysaccharides.

KeywordLentinus Edodes Polysaccharide Qualitative Quantitative Specific Oligosaccharide Uplc-flr-ms
DOI10.1016/j.ijbiomac.2022.02.048
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000782112300002
Scopus ID2-s2.0-85124666413
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Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorZhao, Jing; Li, Shao Ping
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, Macao
2.Joint Laboratory of Chinese Herbal Glycoengineering and Testing Technology, University of Macau, Macao SAR, Macao
First Author AffilicationInstitute of Chinese Medical Sciences;  University of Macau
Corresponding Author AffilicationInstitute of Chinese Medical Sciences;  University of Macau
Recommended Citation
GB/T 7714
Deng, Yong,Chen, Ling Xiao,Zhu, Bao Jie,et al. A quantitative method for polysaccharides based on endo-enzymatic released specific oligosaccharides: A case of Lentinus edodes[J]. International Journal of Biological Macromolecules,2022,205:15-22.
APA Deng, Yong,Chen, Ling Xiao,Zhu, Bao Jie,Zhao, Jing,&Li, Shao Ping.(2022).A quantitative method for polysaccharides based on endo-enzymatic released specific oligosaccharides: A case of Lentinus edodes.International Journal of Biological Macromolecules,205,15-22.
MLA Deng, Yong,et al."A quantitative method for polysaccharides based on endo-enzymatic released specific oligosaccharides: A case of Lentinus edodes".International Journal of Biological Macromolecules 205(2022):15-22.
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