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Design and Implementation of an Automatic Batch Microinjection System for Zebrafish Larvae
Chi, Ziqiang1; Xu, Qingsong1; Ai, Nana2; Ge, Wei2
2022-04-01
Source PublicationIEEE Robotics and Automation Letters
ISSN2377-3766
Volume7Issue:2Pages:1848-1855
Abstract

Microinjection of zebrafish larvae is one of the most common procedures in biomedical experiments. Majority of microinjection work of zebrafish larvae in the laboratory is operated manually. Manual operation is influenced by many factors, and it is difficult to maintain steady efficiency and high success rate. To solve this problem, a novel automated robotic system is proposed for batch microinjection of zebrafish larvae in this letter. For immobilizing the zebrafish larvae in batches with standard posture, an agarose supporting base is specially designed in a Petri dish. It is fabricated to hold the larvae in different orientations, which facilitates a rapid alignment of the randomly placed larvae. To accurately identify the injection targets of zebrafish larvae, a machine vision scheme based on deep learning is established. A prototype microinjection system is developed by integrating the hardware equipment and machine vision software, which is able to complete the injection operation quickly and accurately. The experimental results reveal that the robotic system provides higher injection success rate, better stability, and better performance than those of existing microinjection approaches.

KeywordAutomatic Microinjection System Deep Learning Image Processing Machine Vision Zebrafish Larvae
DOI10.1109/LRA.2022.3143286
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaRobotics
WOS SubjectRobotics
WOS IDWOS:000745524600014
Scopus ID2-s2.0-85123312874
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionDEPARTMENT OF ELECTROMECHANICAL ENGINEERING
Faculty of Health Sciences
Corresponding AuthorXu, Qingsong; Ge, Wei
Affiliation1.Department of Electromechanical Engineering, Faculty of Science and Technology, University of Macau, Macao
2.Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Macao
First Author AffilicationFaculty of Science and Technology
Corresponding Author AffilicationFaculty of Science and Technology;  Centre of Reproduction, Development and Aging
Recommended Citation
GB/T 7714
Chi, Ziqiang,Xu, Qingsong,Ai, Nana,et al. Design and Implementation of an Automatic Batch Microinjection System for Zebrafish Larvae[J]. IEEE Robotics and Automation Letters,2022,7(2):1848-1855.
APA Chi, Ziqiang,Xu, Qingsong,Ai, Nana,&Ge, Wei.(2022).Design and Implementation of an Automatic Batch Microinjection System for Zebrafish Larvae.IEEE Robotics and Automation Letters,7(2),1848-1855.
MLA Chi, Ziqiang,et al."Design and Implementation of an Automatic Batch Microinjection System for Zebrafish Larvae".IEEE Robotics and Automation Letters 7.2(2022):1848-1855.
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