UM  > Faculty of Health Sciences
Residential Collegefalse
Status即將出版Forthcoming
Protective Mechanism of Berberine on Human Retinal Pigment Epithelial Cells against Apoptosis Induced by Hydrogen Peroxide via the Stimulation of Autophagy
Li, Shuai1; Jiang, Yizhou1; Xing, Xingan1; Lin, Ruohong1; Li, Qin2; Zhou, Wenshu1; Qiu, Wei3; Zheng, Wenhua1
2021
Source PublicationOxidative Medicine and Cellular Longevity
ISSN1942-0900
Volume2021
AbstractAge-related macular degeneration (AMD) is a major cause of severe and irreversible vision loss with limited effective therapies. Diminished autophagy and increased oxidative damage caused by ROS in the retinal pigment epithelium (RPE) have been implicated in the pathogenesis of AMD, and strategies aimed at enhancing autophagy are likely to protect these cells from oxidative damage. We have previously shown that berberine (BBR), an isoquinoline alkaloid isolated from Chinese herbs, was able to protect human RPE cells from H2O2-induced oxidative damage through AMPK activation. However, the precise mechanisms behind this protective effect remain unclear. Given the essential role of AMPK in autophagy activation, we postulated that BBR may confer protection against H2O2-induced oxidative damage by stimulating AMPK-dependent autophagy. Our results showed that BBR was able to induce autophagy in D407 cells, whereas autophagy inhibitor PIKIII or silencing of LC3B blocked the protective effect of BBR. Further analysis showed that BBR activated the AMPK/mTOR/ULK1 signaling pathways and that both pharmacological and genetic inhibitions of the AMPK pathway abolished the autophagy-stimulating effect of BBR. Similar results were obtained in primary cultured human RPE cells. Taken together, these results demonstrate that BBR is able to stimulate autophagy in D407 cells via the activation of AMPK pathway and that its protective effect against H2O2-induced oxidative damage relies on its autophagy-modulatory effect. Our findings also provide evidence to support the potential application of BBR in preventing and treating AMD.
DOI10.1155/2021/7654143
URLView the original
Language英語English
Scopus ID2-s2.0-85113760224
Fulltext Access
Citation statistics
Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Center of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Institute of Translation Medicine, Faculty of Health Sciences, University of Macau, Taipa, Macao
2.Hangzhou Medical College, Hangzhou, China
3.Neurology Department, 3rd Affiliated Hospital, Sun Yat-sen University, China
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Li, Shuai,Jiang, Yizhou,Xing, Xingan,et al. Protective Mechanism of Berberine on Human Retinal Pigment Epithelial Cells against Apoptosis Induced by Hydrogen Peroxide via the Stimulation of Autophagy[J]. Oxidative Medicine and Cellular Longevity,2021,2021.
APA Li, Shuai,Jiang, Yizhou,Xing, Xingan,Lin, Ruohong,Li, Qin,Zhou, Wenshu,Qiu, Wei,&Zheng, Wenhua.(2021).Protective Mechanism of Berberine on Human Retinal Pigment Epithelial Cells against Apoptosis Induced by Hydrogen Peroxide via the Stimulation of Autophagy.Oxidative Medicine and Cellular Longevity,2021.
MLA Li, Shuai,et al."Protective Mechanism of Berberine on Human Retinal Pigment Epithelial Cells against Apoptosis Induced by Hydrogen Peroxide via the Stimulation of Autophagy".Oxidative Medicine and Cellular Longevity 2021(2021).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Shuai]'s Articles
[Jiang, Yizhou]'s Articles
[Xing, Xingan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Shuai]'s Articles
[Jiang, Yizhou]'s Articles
[Xing, Xingan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Shuai]'s Articles
[Jiang, Yizhou]'s Articles
[Xing, Xingan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.