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Qingwei San treats oral ulcer subjected to stomach heat syndrome in db/db mice by targeting TLR4/MyD88/NF-κB pathway
Shi, Lu1; An, Yongcheng2; Cheng, Long1; Li, Yiyang3; Li, Huimin1; Wang, Chen2; Lv, Yinglan1; Duan, Yuhui1; Dai, Hongyu1; He, Changhao1; Zhang, Huilin1; Huang, Yan2; Fu, Wanxin2; Wang, Shengpeng3; Zhao, Baosheng4; Wang, Yitao3; Zhao, Yonghua3
2022-01-04
Source PublicationChinese Medicine
ISSN1749-8546
Volume17Issue:1Pages:1
Abstract

Background: Qingwei San (QWS), one of classic Chinese Medicine prescripts, has been widely used to treat stomach heat syndrome which manifests oral ulcer (OU), periodontitis and upper gastrointestinal bleeding for seven hundred years. However, the therapeutic effects of QWS on diabetic OU subjected to stomach heat syndrome are still ambiguous. In the study, we investigated the pharmacological mechanisms. Methods: The main components of QWS aqueous extract were analyzed by LC–MS, and potential pathways of QWS targeting OU were predicted by network pharmacology. The db/db mice were administered with the decoction of dried Zingiber officinale Rosc. rhizome combined with NaOH cauterization to establish the model of diabetic OU subjected to stomach heat syndrome. Subsequently, the model mice were treated with QWS, and OU wound healing status were recorded. The pathological changes of gastric tissue and oral mucosa were evaluated using hematoxylin–eosin staining, and the morphology of collagen fibers in oral mucosa was assessed by Masson staining. The levels of thromboxane B (TXB), 6-Keto-prostaglandin F1α (6-keto-PGF1α), interleukin-1 β (IL-1β), IL-2, IL-6, tumor necrosis factor-α (TNF-α), β-endorphin (β-EP) and 5-Hydroxytryptamine (5-HT) were determined by ELISA assay. The protein expressions of Toll-like receptor 4 (TLR4), TNF receptor associated factor 6 (TRAF6), myeloid differentiation factor 88 (MyD88), inhibitor of NF-κB alpha (IκΒα), p-IκΒα and nuclear factor kappa-B (NF-κB) p65 were measured by Western Blotting. Results: A total of 183 compounds in QWS were identified by LC–MS, and identified 79 bioactive compounds corresponded to 269 targets and 59 pathways. QWS high-dose treatment significantly reduced the level of TXB and the ratio of TXB/6-keto-PGF1α. Meanwhile, it improved mucosal pathological morphology, and reduced the area of OU and local edema. Simultaneously, the levels of TNF-α, IL-1β, IL-6, IL-2 and 5-HT, and the expressions of TLR4, TRAF6, MyD88, p-IκΒα and NF-κB p65 were decreased. Conclusion: QWS treatment facilitates the healing of OU, ameliorates pathological morphologies of gastric and oral mucosa and decreases the levels of pro-inflammatory cytokines in db/db mice subjected to stomach heat syndrome, whose mechanism may be associated with the inhibition of TLR4/MyD88/NF-κB signaling pathway to exert anti-inflammatory effects.

KeywordDiabetes Mellitus Oral Ulcer Qingwei San Stomach Heat Syndrome Tlr4/myd88/nf-κb
DOI10.1186/s13020-021-00565-5
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS SubjectIntegrative & Complementary Medicinepharmacology & Pharmacy
WOS IDWOS:000737980600001
Scopus ID2-s2.0-85122183242
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Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorZhao, Baosheng; Zhao, Yonghua
Affiliation1.Department of Pharmacology, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, China
2.School of Life Sciences, Beijing University of Chinese Medicine, Beijing, 102488, China
3.Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Research Building N22, Avenida da Universidade, Taipa, 999078, Macao
4.Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, No. 11 North 3rd Ring East Road, Chao-Yang District, 100029, China
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Shi, Lu,An, Yongcheng,Cheng, Long,et al. Qingwei San treats oral ulcer subjected to stomach heat syndrome in db/db mice by targeting TLR4/MyD88/NF-κB pathway[J]. Chinese Medicine,2022,17(1):1.
APA Shi, Lu,An, Yongcheng,Cheng, Long,Li, Yiyang,Li, Huimin,Wang, Chen,Lv, Yinglan,Duan, Yuhui,Dai, Hongyu,He, Changhao,Zhang, Huilin,Huang, Yan,Fu, Wanxin,Wang, Shengpeng,Zhao, Baosheng,Wang, Yitao,&Zhao, Yonghua.(2022).Qingwei San treats oral ulcer subjected to stomach heat syndrome in db/db mice by targeting TLR4/MyD88/NF-κB pathway.Chinese Medicine,17(1),1.
MLA Shi, Lu,et al."Qingwei San treats oral ulcer subjected to stomach heat syndrome in db/db mice by targeting TLR4/MyD88/NF-κB pathway".Chinese Medicine 17.1(2022):1.
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