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Sigesbeckia orientalis L. Derived Active Fraction Ameliorates Perioperative Neurocognitive Disorders Through Alleviating Hippocampal Neuroinflammation
Chu, John Man Tak1; Abulimiti, Amina1; Wong, Brian Shing Hei1; Zhao, Guan Ding2; Xiong, Shi Hang2; Zhao, Ming Ming2; Wang, Yingyi1; Chen, Ying1; Wang, Jiaqi1; Zhang, Yan1; Chang, Raymond Chuen Chung3,4; Yu, Hua2; Wong, Gordon Tin Chun1
2022-03-17
Source PublicationFrontiers in Pharmacology
Volume13
Abstract

Neuroinflammation is closely related to the pathogenesis of perioperative neurocognitive disorders (PNDs), which is characterized by the activation of microglia, inflammatory pathways and the release of inflammatory mediators. Sigesbeckia orientalis L. (SO) is a traditional Chinese medicine which demonstrates anti-inflammatory activities in different models. In this study, we aim to isolate the active fraction from the extract of SO with higher anti-inflammatory potential and confirm if the selected fraction exerts neuroprotection against the development of PND in an animal model. Moreover, the components in the selected fraction would be determined by UPLC-PDA analysis. Three fractions were prepared by column chromatography packed with three different macroporous resins. Anti-inflammatory activities of prepared fractions were accessed in microglial BV2 cultures by nitric oxide release, gene expression of inflammatory cytokines and activation of inflammatory JNK and NF-kB pathway molecules. Our results demonstrated that the fraction prepared from D101 macroporous resin (D101 fraction) exhibited a more potent anti-neuroinflammatory effect. The neuroprotective effect of D101 fraction was further examined in postoperative mice. Our results showed that surgery-induced cognitive dysfunction was attenuated by the D101 fraction treatment. This fraction also reduced microglial activation, inflammatory cytokines and inhibiting JNK and NF-kB pathway molecules in the hippocampus. In addition, surgery induced dendritic spine loss while D101 fraction ameliorated the spine loss in the hippocampus. For safety concerns, anti-thrombotic effect was examined by tail bleeding assay and no significant change of the bleeding pattern was found. UPLC-PDA analysis indicated that flavonoids (rutin, isochlorogenic acid A, isochlorogenic acid C) and terpenoid (darutoside) were the most important components in the D101 fraction. Our results support a therapeutic, as well as the translational potential for D101 fraction in ameliorating postoperative neuroinflammation and subsequent PND in the clinical setting without increasing bleeding tendencies.

KeywordMicroglia Neuroinflammation Perioperative Neurocognitive Disorders Sigesbeckia Orientalis l Surgery
DOI10.3389/fphar.2022.846631
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000777999000001
Scopus ID2-s2.0-85127867196
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYu, Hua; Wong, Gordon Tin Chun
Affiliation1.Department of Anaesthesiology, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong
2.Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, The University of Macau, Taipa, China
3.Laboratory of Neurodegenerative Diseases, School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong
4.The State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong, Hong Kong
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Chu, John Man Tak,Abulimiti, Amina,Wong, Brian Shing Hei,et al. Sigesbeckia orientalis L. Derived Active Fraction Ameliorates Perioperative Neurocognitive Disorders Through Alleviating Hippocampal Neuroinflammation[J]. Frontiers in Pharmacology,2022,13.
APA Chu, John Man Tak,Abulimiti, Amina,Wong, Brian Shing Hei,Zhao, Guan Ding,Xiong, Shi Hang,Zhao, Ming Ming,Wang, Yingyi,Chen, Ying,Wang, Jiaqi,Zhang, Yan,Chang, Raymond Chuen Chung,Yu, Hua,&Wong, Gordon Tin Chun.(2022).Sigesbeckia orientalis L. Derived Active Fraction Ameliorates Perioperative Neurocognitive Disorders Through Alleviating Hippocampal Neuroinflammation.Frontiers in Pharmacology,13.
MLA Chu, John Man Tak,et al."Sigesbeckia orientalis L. Derived Active Fraction Ameliorates Perioperative Neurocognitive Disorders Through Alleviating Hippocampal Neuroinflammation".Frontiers in Pharmacology 13(2022).
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