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Psoralidin, a natural compound from Psoralea corylifolia, induces oxidative damage mediated apoptosis in colon cancer cells
Sun, Chong1,2; Zhao, Lin1,2; Wang, Xianzhe1,2; Hou, Ying1,2; Guo, Xiuli3; Lu, Jin Jian1,2; Chen, Xiuping1,2
2022-03
Source PublicationJournal of Biochemical and Molecular Toxicology
ISSN1095-6670
Abstract

Psoralidin (PSO) is a natural coumarin isolated from the seeds of Psoralea corylifolia Linn. Previous studies have reported that PSO exerts numerous pharmacological bioactivities including antitumor. The present study aimed to investigate its anticancer effect using colon cancer cells. Cultured HT-29 and HCT-116 colon cancer cells were treated with different concentrations of PSO, and the cell viability, the intracellular reactive oxygen species (ROS), the protein expression, and the apoptosis were determined by MTT assay, DCFH-DA fluorescence probe, Western blotting, and Annexin V/7-AAD staining, respectively. The activities of caspase 3/7 were determined by a commercial kit. Our study found that PSO effectively induces apoptotic cell death mediated by caspase 3/7 in HT-29 and HCT-116 colon cancer cells. PSO treatment rapidly boosts the ROS generation, which is responsible for the PSO-triggered DNA damage, mitochondria membrane potential decrease and caspase 3/7 activation, and c-Jun N-terminal kinase 1/2 activation. Collectively, these results showed that PSO triggered oxidative damage mediated apoptosis in colon cancer cells.

KeywordApoptosis Cell Death Ros Chinese Medicine
DOI10.1002/jbt.23051
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology ; Toxicology
WOS SubjectBiochemistry & Molecular Biology ; Toxicology
WOS IDWOS:000771390200001
Scopus ID2-s2.0-85126784601
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Document TypeJournal article
CollectionDEPARTMENT OF PHARMACEUTICAL SCIENCES
Institute of Chinese Medical Sciences
THE STATE KEY LABORATORY OF QUALITY RESEARCH IN CHINESE MEDICINE (UNIVERSITY OF MACAU)
Corresponding AuthorChen, Xiuping
Affiliation1.Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao
2.Department of Pharmaceutical Sciences, Faculty of Health Sciences, University of Macau, Macao
3.Department of Pharmacology, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan, China
First Author AffilicationInstitute of Chinese Medical Sciences;  Faculty of Health Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences;  Faculty of Health Sciences
Recommended Citation
GB/T 7714
Sun, Chong,Zhao, Lin,Wang, Xianzhe,et al. Psoralidin, a natural compound from Psoralea corylifolia, induces oxidative damage mediated apoptosis in colon cancer cells[J]. Journal of Biochemical and Molecular Toxicology,2022.
APA Sun, Chong,Zhao, Lin,Wang, Xianzhe,Hou, Ying,Guo, Xiuli,Lu, Jin Jian,&Chen, Xiuping.(2022).Psoralidin, a natural compound from Psoralea corylifolia, induces oxidative damage mediated apoptosis in colon cancer cells.Journal of Biochemical and Molecular Toxicology.
MLA Sun, Chong,et al."Psoralidin, a natural compound from Psoralea corylifolia, induces oxidative damage mediated apoptosis in colon cancer cells".Journal of Biochemical and Molecular Toxicology (2022).
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