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High Expression of G6PD Increases Doxorubicin Resistance in Triple Negative Breast Cancer Cells by Maintaining GSH Level
Luo, Man1,2; Fu, Afu2; Wu, Renfei3; Wei, Na2; Song, Kai4; Lim, Sierin2; Luo, Kathy Qian3,5
2022-01
Source PublicationInternational journal of biological sciences
ISSN1449-2288
Volume18Issue:3Pages:1120-1133
Abstract

Resistance to doxorubicin (DOX) remains a big challenge to breast cancer treatment especially for triple negative breast cancer (TNBC). Our previous study revealed that the antioxidant system plays an important role in conferring metastasis derived DOX resistance. In this study, we used two-dimensional difference gel electrophoresis (2D-DIGE) proteomics to compare the expression profiles of two generations of TNBC cell lines which have increased metastatic ability in nude mice and exhibited resistance to DOX. Through careful analyses, one antioxidant protein: glucose-6-phosphate dehydrogenase (G6PD) was identified with 3.2-fold higher level in metastatic/DOX-resistant 231-M1 than its parental 231-C3 cells. Analyses of clinical data showed that TNBC patients with higher G6PD levels exhibited lower overall survival than patients with lower G6PD level. Reducing G6PD expression by siRNA or inhibiting its activity with dehydroepiandrosterone (DHEA) significantly increased DOX's cytotoxicity in both cell lines. Importantly, inhibiting G6PD's activity with DHEA dramatically increased the apoptotic rate of 1.25 µM DOX from 2% to 54%. Our results suggest that high level of G6PD can help TNBC to resist DOX-induced oxidative stress. Thus, inhibiting G6PD shall be a good strategy to treat DOX-resistant TNBC.

KeywordDoxorubicin Drug Resistance G6pd Metastasis Oxidative Stress Triple Negative Breast Cancer
DOI10.7150/ijbs.65555
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS IDWOS:000744549300001
Scopus ID2-s2.0-85124778197
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorLuo, Kathy Qian
Affiliation1.Interdisciplinary Graduate School, Nanyang Technological University, Singapore
2.School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore
3.Faculty of Health Sciences, University of Macau, China
4.Department of Bioengineering, University of California, Los Angeles, CA, USA
5.Ministry of Education-Frontiers Science Center for Precision Oncology, University of Macau, Taipa, China
Corresponding Author AffilicationFaculty of Health Sciences;  University of Macau
Recommended Citation
GB/T 7714
Luo, Man,Fu, Afu,Wu, Renfei,et al. High Expression of G6PD Increases Doxorubicin Resistance in Triple Negative Breast Cancer Cells by Maintaining GSH Level[J]. International journal of biological sciences,2022,18(3):1120-1133.
APA Luo, Man,Fu, Afu,Wu, Renfei,Wei, Na,Song, Kai,Lim, Sierin,&Luo, Kathy Qian.(2022).High Expression of G6PD Increases Doxorubicin Resistance in Triple Negative Breast Cancer Cells by Maintaining GSH Level.International journal of biological sciences,18(3),1120-1133.
MLA Luo, Man,et al."High Expression of G6PD Increases Doxorubicin Resistance in Triple Negative Breast Cancer Cells by Maintaining GSH Level".International journal of biological sciences 18.3(2022):1120-1133.
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