Residential College | false |
Status | 已發表Published |
The role of BMP-4 and GATA-2 in the induction and differentiation of hematopoietic mesoderm in Xenopus laevis | |
Mitsugu Maeno1; Paul E. Mead; Clair Kelley; Ren-he Xu; Hsiang-fu Kung; Atsushi Suzuki; Naoto Ueno; Leonard I. Zon | |
1996-09-15 | |
Source Publication | Blood |
ISSN | 0006-4971 |
Volume | 88Issue:6Pages:1965-1972 |
Abstract | ertebrate embryonic blood formation is regulated by factors that participate in dorsal-ventral patterning and mesoderm induction. The GATA-binding transcription factors are required for normal hematopoiesis and are expressed during gastrulation when ventral mesoderm (VM) is induced to form blood. Based on the recent demonstration that bone morphogenetic protein (BMP-4) is a potent ventralizing factor and inducer of hematopoietic tissue, we hypothesized that GATA-2 could be induced or activated by BMP-4. Here we demonstrate that BMP-4 can stimulate GATA-2 expression, and that expression of a dominant negative BMP-4 receptor can suppress GATA-2 induction by BMP-4 in ventral mesoderm. Over-expression of GATA-2 in ventral mesoderm leads to increased globin production and forced expression of GATA-2 in primitive ectoderm adjacent to ventral mesoderm also stimulates globin expression. Our results suggest that BMP-4 and GATA-2 can function in two adjacent germ layers, mesoderm and ectoderm, to participate in blood cell formation during embryogenesis. |
DOI | 10.1182/blood.V88.6.1965.bloodjournal8861965 |
URL | View the original |
Indexed By | SCIE |
Language | 英語English |
WOS Research Area | Hematology |
WOS Subject | Hematology |
WOS ID | WOS:A1996VK56500008 |
Scopus ID | 2-s2.0-0029793060 |
Fulltext Access | |
Citation statistics | |
Document Type | Journal article |
Collection | Personal research not belonging to the institution |
Affiliation | 1.Department of Biology, Faculty of Science, Niigata Universiq, Niigata, Japan 2.the Division of Hematology, Department of Pediatrics, Howard Hughes Medical Institute, Children’s Hospital, Boston, M 3.the Laboratovy of Biochemical Physiology, Department of Basic Sciences, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, MD 4.the Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, Japan |
Recommended Citation GB/T 7714 | Mitsugu Maeno,Paul E. Mead,Clair Kelley,et al. The role of BMP-4 and GATA-2 in the induction and differentiation of hematopoietic mesoderm in Xenopus laevis[J]. Blood,1996,88(6):1965-1972. |
APA | Mitsugu Maeno,Paul E. Mead,Clair Kelley,Ren-he Xu,Hsiang-fu Kung,Atsushi Suzuki,Naoto Ueno,&Leonard I. Zon.(1996).The role of BMP-4 and GATA-2 in the induction and differentiation of hematopoietic mesoderm in Xenopus laevis.Blood,88(6),1965-1972. |
MLA | Mitsugu Maeno,et al."The role of BMP-4 and GATA-2 in the induction and differentiation of hematopoietic mesoderm in Xenopus laevis".Blood 88.6(1996):1965-1972. |
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